Abstract

The purpose of the large animal core will be to coordinate and facilitate the in utero gene therapy protocols of all investigators. The personnel supported by this core have extensive experience in general sheep husbandry and in specific aspects of the sheep as an experimental model. This includes extensive experience in survival surgical manipulation of the fetus, with implantation of chronically indwelling fetal catheters and blood flow probes. In the past year we have also developed expertise in surgical approach to 50 day fetuses. The services that the core will provide are: 1. Coordination of all aspects of animal care. 2. Performance of all Surgical Procedures. 3. Post operative Care of transplanted animals. 4. Supervising lambing procedures. The demands on the core as proposed in this grant (12 sheep per year) are well within the capabilities of present personnel and facilities. Therefore, prioritization will involve only the scheduling of surgical time for cell transplantation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Specialized Center (P50)
Project #
5P50DK049218-03
Application #
5210901
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Song, Liujiang; Kauss, M Ariel; Kopin, Etana et al. (2013) Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivo. Cytotherapy 15:986-98
Jin, Qingwen; Marsh, Jon; Cornetta, Kenneth et al. (2008) Resistance to human immunodeficiency virus type 1 (HIV-1) generated by lentivirus vector-mediated delivery of the CCR5{Delta}32 gene despite detectable expression of the HIV-1 co-receptors. J Gen Virol 89:2611-21
Case, Jamie; Horvath, Tamara L; Ballas, Christopher B et al. (2008) In vitro clonal analysis of murine pluripotent stem cells isolated from skeletal muscle and adipose stromal cells. Exp Hematol 36:224-34
Zhang, Shangming; Joseph, Guiandre; Pollok, Karen et al. (2006) G2 cell cycle arrest and cyclophilin A in lentiviral gene transfer. Mol Ther 14:546-54
Goebel, W Scott; Mark, Lawrence A; Billings, Steven D et al. (2005) Gene correction reduces cutaneous inflammation and granuloma formation in murine X-linked chronic granulomatous disease. J Invest Dermatol 125:705-10
Case, Jamie; Horvath, Tamara L; Howell, Jonathan C et al. (2005) Clonal multilineage differentiation of murine common pluripotent stem cells isolated from skeletal muscle and adipose stromal cells. Ann N Y Acad Sci 1044:183-200
Cornetta, K; Matheson, L; Ballas, C (2005) Retroviral vector production in the National Gene Vector Laboratory at Indiana University. Gene Ther 12 Suppl 1:S28-35
Broxmeyer, Hal E; Orschell, Christie M; Clapp, D Wade et al. (2005) Rapid mobilization of murine and human hematopoietic stem and progenitor cells with AMD3100, a CXCR4 antagonist. J Exp Med 201:1307-18
Kahl, Christoph A; Pollok, Karen; Haneline, Laura S et al. (2005) Lentiviral vectors pseudotyped with glycoproteins from Ross River and vesicular stomatitis viruses: variable transduction related to cell type and culture conditions. Mol Ther 11:470-82
Sastry, Lakshmi; Xu, Yi; Duffy, Lisa et al. (2005) Product-enhanced reverse transcriptase assay for replication-competent retrovirus and lentivirus detection. Hum Gene Ther 16:1227-36

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