The Analytical Core provides support to Center Investigators for the application of modern methods of synthesis and analysis to their projects. The Core also functions as a resource in the development of new analytical procedures, with particular emphasis on the use of mass spectrometry. Methods that are available for structural analysis include fast atom bombardment mass spectrometry, desorption chemical ionization, high resolution mass spectrometry and linked scanning techniques and high resolution gas chromatography mass spectrometry. Quantitative analysis of trace amounts of complex lipid-derived mediators can be carried out using highly sensitive methods based on stable isotope dilution negative ion chemical ionization mass spectrometry. The application of these diverse methodologies will greatly enhance the ability of the Center Investigators to carry out their proposed projects. It is envisaged that during the course of their proposed studies new exciting directions of research will emerge. The Analytical Core will provide expertise that will help to facilitate such possibilities. Furthermore, the presence of a well equipped synthetic laboratory will allow the synthesis of otherwise unavailable compounds to be carried out. The Analytical Core has in the past developed analytical methods, carried out structural studies and has enabled Center Investigators to access highly sophisticated instrumentation. In addition, it has provided Center Investigators with a number of otherwise inaccessible synthetic standards. It is envisaged that the Core will continue to function in these roles in the future.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM015431-22
Application #
3898170
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
22
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37203
Salisbury-Ruf, Christi T; Bertram, Clinton C; Vergeade, Aurelia et al. (2018) Bid maintains mitochondrial cristae structure and function and protects against cardiac disease in an integrative genomics study. Elife 7:
Kong, Deping; Li, Juanjuan; Shen, Yujun et al. (2017) Niacin Promotes Cardiac Healing after Myocardial Infarction through Activation of the Myeloid Prostaglandin D2 Receptor Subtype 1. J Pharmacol Exp Ther 360:435-444
Teder, Tarvi; Boeglin, William E; Brash, Alan R (2017) Oxidation of C18 Hydroxy-Polyunsaturated Fatty Acids to Epoxide or Ketone by Catalase-Related Hemoproteins Activated with Iodosylbenzene. Lipids 52:587-597
Plewes, Katherine; Kingston, Hugh W F; Ghose, Aniruddha et al. (2017) Cell-free hemoglobin mediated oxidative stress is associated with acute kidney injury and renal replacement therapy in severe falciparum malaria: an observational study. BMC Infect Dis 17:313
Kudalkar, Shalley N; Kingsley, Philip J; Marnett, Lawrence J (2016) Assay of Endocannabinoid Oxidation by Cyclooxygenase-2. Methods Mol Biol 1412:205-15
Wu, Jing; Montaniel, Kim Ramil C; Saleh, Mohamed A et al. (2016) Origin of Matrix-Producing Cells That Contribute to Aortic Fibrosis in Hypertension. Hypertension 67:461-8
Wu, Jing; Saleh, Mohamed A; Kirabo, Annet et al. (2016) Immune activation caused by vascular oxidation promotes fibrosis and hypertension. J Clin Invest 126:50-67
Mashhadi, Zahra; Newcomer, Marcia E; Brash, Alan R (2016) The Thr-His Connection on the Distal Heme of Catalase-Related Hemoproteins: A Hallmark of Reaction with Fatty Acid Hydroperoxides. Chembiochem 17:2000-2006
Kong, Deping; Shen, Yujun; Liu, Guizhu et al. (2016) PKA regulatory II? subunit is essential for PGD2-mediated resolution of inflammation. J Exp Med 213:2209-26
Boutaud, Olivier; Sosa, I Romina; Amin, Taneem et al. (2016) Inhibition of the Biosynthesis of Prostaglandin E2 By Low-Dose Aspirin: Implications for Adenocarcinoma Metastasis. Cancer Prev Res (Phila) 9:855-865

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