Trauma is the leading cause of death In this country in people under the age of 44 years. After major head injury, hemorrhage and its consequences are the most important causes of morbidity and mortality in these young, otherwise healthy individuals. This Center Grant proposal seeks to advance our understanding of the molecular mechanisms leading to organ injury and dysfunction in the severest forms of the hemorrhagic insult. By studying the rapid onset of organ injury, which is characteristic of severe hemorrhagic shock, the P.I. will exclude other delayed causes of morbidity such as sepsis due to infection, commonly encountered in trauma victims but also other critically ill patients. Stated another way, the P.I. believes the pathophysiology of the tissue injury and organ failure associated with hemorrhagic shock alone is a poorly understood and complex process that warrants focused and detailed study. Furthermore, a better understanding of this disease process will lead to more effective treatment strategies. The P.I. proposes that one of the keys to understanding organ injury resulting from hemorrhagic shock is to characterize the molecular events leading to the initiation and promotion of inflammatory changes in hemorrhagic shock. Most previous studies have examined the net effect of shock plus resuscitation on parameters of inflammation and organ damage. In contrast to these previous approaches, the P.I. divides the molecular events of the hemorrhagic shock into three distinct phases: the shock phase, the resuscitation phase, and the post-resuscitation phase. The P.I. hypothesizes that there are specific changes in gene expression in each phase that are induced by the effects of shock alone or the consequences of resuscitation following shock. These induced changes in gene expression promote inflammation and organ injury. The P.I. postulates that the duration and severity of the shock phase determine the degree of phenotypic changes and hence the intensity of the inflammatory response following resuscitation. The P.I. also proposes that the susceptibility of the host to subsequent inflammatory insults is enhanced by changes in gene expression resulting from the shock and resuscitation phases, and that this enhanced vulnerability prevents the resolution of the inflammatory response.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM053789-02
Application #
2713745
Study Section
Special Emphasis Panel (ZGM1-TB-4 (01))
Project Start
1997-06-01
Project End
2000-05-31
Budget Start
1998-06-01
Budget End
1999-05-31
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Surgery
Type
Schools of Medicine
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
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Zettel, Kent; Korff, Sebastian; Zamora, Ruben et al. (2017) Toll-Like Receptor 4 on both Myeloid Cells and Dendritic Cells Is Required for Systemic Inflammation and Organ Damage after Hemorrhagic Shock with Tissue Trauma in Mice. Front Immunol 8:1672
Sun, Qian; Loughran, Patricia; Shapiro, Richard et al. (2017) Redox-dependent regulation of hepatocyte absent in melanoma 2 inflammasome activation in sterile liver injury in mice. Hepatology 65:253-268
Zettel, Kent R; Dyer, Mitchell; Raval, Jay S et al. (2017) Aged Human Stored Red Blood Cell Supernatant Inhibits Macrophage Phagocytosis in an HMGB1 Dependent Manner After Trauma in a Murine Model. Shock 47:217-224
Moore, Frederick A; Moore, Ernest E; Billiar, Timothy R et al. (2017) The role of NIGMS P50 sponsored team science in our understanding of multiple organ failure. J Trauma Acute Care Surg 83:520-531
Yang, Jie; Zhao, Yanfeng; Zhang, Peng et al. (2016) Hemorrhagic shock primes for lung vascular endothelial cell pyroptosis: role in pulmonary inflammation following LPS. Cell Death Dis 7:e2363
Namas, Rami A; Almahmoud, Khalid; Mi, Qi et al. (2016) Individual-specific principal component analysis of circulating inflammatory mediators predicts early organ dysfunction in trauma patients. J Crit Care 36:146-153
Korff, Sebastian; Loughran, Patricia; Cai, Changchun et al. (2016) Tlr2 on Bone Marrow and Non-Bone Marrow Derived Cells Regulates Inflammation and Organ Injury in Cooperation with Tlr4 During Resuscitated Hemorrhagic Shock. Shock 46:519-526
Li, Z; Scott, M J; Fan, E K et al. (2016) Tissue damage negatively regulates LPS-induced macrophage necroptosis. Cell Death Differ 23:1428-47
Namas, Rami A; Vodovotz, Yoram; Almahmoud, Khalid et al. (2016) Temporal Patterns of Circulating Inflammation Biomarker Networks Differentiate Susceptibility to Nosocomial Infection Following Blunt Trauma in Humans. Ann Surg 263:191-8

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