Abstract

Comparison of genomic sequences of several organisms with sequence databases reveals that a large fraction of proteins coded by the genes have not known functions and/or structures. Structures of these proteins may provide additional key information for the discovery of the molecular functions of these proteins and the relationship with their three-dimensional folds. Three-dimensional structures of the majority of the proteins from a minimal genome (Mycoplasma genitalium/pneumoniae) or their homologues, for which no functions and/or structures are known, will be determined by X-ray crystallographic methods.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
5P50GM062412-02
Application #
6495129
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
$1,094,371
Indirect Cost

  Institution

Name
University of California Berkeley
Department
Type
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704

  Publications

Jun, Se-Ran; Sims, Gregory E; Wu, Guohong A et al. (2010) Whole-proteome phylogeny of prokaryotes by feature frequency profiles: An alignment-free method with optimal feature resolution. Proc Natl Acad Sci U S A 107:133-8
Lee, Jonas; Kim, Sung-Hou (2009) High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences. Protein Expr Purif 63:58-61
Wu, Guohong Albert; Jun, Se-Ran; Sims, Gregory E et al. (2009) Whole-proteome phylogeny of large dsDNA virus families by an alignment-free method. Proc Natl Acad Sci U S A 106:12826-31
Pereira, Jose Henrique; Kim, Sung-Hou (2009) Structure of human Brn-5 transcription factor in complex with CRH gene promoter. J Struct Biol 167:159-65
Sims, Gregory E; Jun, Se-Ran; Wu, Guohong Albert et al. (2009) Whole-genome phylogeny of mammals: evolutionary information in genic and nongenic regions. Proc Natl Acad Sci U S A 106:17077-82
Sims, Gregory E; Jun, Se-Ran; Wu, Guohong A et al. (2009) Alignment-free genome comparison with feature frequency profiles (FFP) and optimal resolutions. Proc Natl Acad Sci U S A 106:2677-82
Pereira, Jose Henrique; Ha, Sung Chul; Kim, Sung-Hou (2008) Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA. Acta Crystallogr Sect F Struct Biol Cryst Commun 64:175-8
Dahl, Christiane; Schulte, Andrea; Stockdreher, Yvonne et al. (2008) Structural and molecular genetic insight into a widespread sulfur oxidation pathway. J Mol Biol 384:1287-300
Shin, Dong Hae (2008) Preliminary structural studies on MPN423 expressed from an orthologous ORFan of Mycoplasma pneumoniae. Protein Pept Lett 15:753-5
Zwart, Peter H; Afonine, Pavel V; Grosse-Kunstleve, Ralf W et al. (2008) Automated structure solution with the PHENIX suite. Methods Mol Biol 426:419-35

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