The proposed Center of Excellence in Genome Science will develop and apply novel tools for studying natural genetic variation. Following the imminent completion of reference sequences for the genomes of intensively studied organisms, a major future direction of genome research will be to analyze natural variation in genome sequences. This variation underlies genetic individual and the evolutionary process of speciation. The proposed Center will addressed tow major obstacles to the biological interpretation of genetic variation. First, it will develop efficient laboratory methods, based on recombinant-DNA techniques, to resequence long, targeted segments (ranging in length from 10-1000kbp) of any genome from multiple individuals. This capability will address a major current limitation in genetic variation since existing technology, which is largely based on the polymerase-chain reaction, is difficult to apply even to regions the size of a typical human gene (10-100 kbp). Second, the Center will collaborate closely with leading statistical geneticists in order to couple the Center's technology will be applicable to any organism, the Center's own applications will largely be to human genes. These applications are directed toward developing an improved understanding of the molecular basis of known genetic susceptibilities to type I diabetes, progressive supranuclear palsy, and neutropenia. More broadly, the Center expects, through its development of new technology and improved synergy between theory and experiment, to contribute indirectly to many research projects directed at understanding the genetic contributions to human health and disease.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Specialized Center (P50)
Project #
1P50HG002351-01
Application #
6506571
Study Section
National Human Genome Research Institute Initial Review Group (GNOM)
Project Start
2001-09-01
Project End
2006-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Lambert, Charla A; Connelly, Caitlin F; Madeoy, Jennifer et al. (2010) Highly punctuated patterns of population structure on the X chromosome and implications for African evolutionary history. Am J Hum Genet 86:34-44
Spencer, David H; Bubb, Kerry L; Olson, Maynard V (2006) Detecting disease-causing mutations in the human genome by haplotype matching. Am J Hum Genet 79:958-64
Bubb, K L; Bovee, D; Buckley, D et al. (2006) Scan of human genome reveals no new Loci under ancient balancing selection. Genetics 173:2165-77
Smith, Eric E; Buckley, Danielle G; Wu, Zaining et al. (2006) Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients. Proc Natl Acad Sci U S A 103:8487-92
Li, Ruiqiang; Ye, Jia; Li, Songgang et al. (2005) ReAS: Recovery of ancestral sequences for transposable elements from the unassembled reads of a whole genome shotgun. PLoS Comput Biol 1:e43
Yu, Jun; Wang, Jun; Lin, Wei et al. (2005) The Genomes of Oryza sativa: a history of duplications. PLoS Biol 3:e38
Raymond, Christopher K; Subramanian, Sandhya; Paddock, Marcia et al. (2005) Targeted, haplotype-resolved resequencing of long segments of the human genome. Genomics 86:759-66
Raymond, Christopher K; Kas, Arnold; Paddock, Marcia et al. (2005) Ancient haplotypes of the HLA Class II region. Genome Res 15:1250-7
Xia, Qingyou; Zhou, Zeyang; Lu, Cheng et al. (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306:1937-40
Olson, M V; Kas, A; Bubb, K et al. (2004) Hypervariability, suppressed recombination and the genetics of individuality. Philos Trans R Soc Lond B Biol Sci 359:129-40

Showing the most recent 10 out of 12 publications