This core laboratory will provide peptide and protein antigens, and will use these antigens for the production of polyclonal antibodies. The core facility has an Applied Biosystems Model 431 A peptide synthesizer capable of both FMOC and tBOC methods, and HF Apparatus for cleaving synthetic peptides from resin using anhydrous hydrogen fluoride, a high pressure liquid chromatograph with autoinjector and diode array detector for amino acid analysis and analytical reverse phase HPLC of peptides, and standard chromatography and electrophoresis equipment for preparation of apolipoproteins and antibody purification.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL015062-23
Application #
3757862
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
23
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637
Tsao, R; Jones, S A; Giddens, D P et al. (1995) An automated three-dimensional particle tracking technique for the study of modeled arterial flow fields. J Biomech Eng 117:211-8