Core - Humoral and Cellular Mediators of Lung Injury

Blair, Ian A.

Abstract

This project includes the quantitative analysis of eicosanoids and """"""""platelet activating factor"""""""" (PAF) from in vitro and in vivo experimental preparations. The accurate purification and quantification of PAF and eicosanoids released in vivo into sheep lung lymph and bronchoalveolar lavage fluid following exposure of animals to endotoxin, hyperoxia or thoracic radiation are necessary to Section III, Projects A, B and C. Included within this project are the development of highly sophisticated analytic techniques which will allow us to accurately quantify eicosanoids, PAF and new mediators as they are identified. These studies will help to define the role of lipid mediators in a variety of lung injuries and help to formulate logical and efficacious methods for the future study and therapy of diffuse lung injury.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Specialized Center (P50)
• Project #: 2P50HL019153-11
• Application #: 3943692
• Support Year: 11
• Fiscal Year: 1987

Institution

• Name: Vanderbilt University Medical Center
• DUNS #: 004413456
• City: Nashville
• State: TN
• Country: United States
• Zip Code: 37203

Publications

Brigham, K L; Lane, K B; Meyrick, B et al. (2000) Transfection of nasal mucosa with a normal alpha1-antitrypsin gene in alpha1-antitrypsin-deficient subjects: comparison with protein therapy. Hum Gene Ther 11:1023-32

Conner, B D; Bernard, G R (2000) Acute respiratory distress syndrome. Potential pharmacologic interventions. Clin Chest Med 21:563-87

Mangialardi, R J; Martin, G S; Bernard, G R et al. (2000) Hypoproteinemia predicts acute respiratory distress syndrome development, weight gain, and death in patients with sepsis. Ibuprofen in Sepsis Study Group. Crit Care Med 28:3137-45

Brigham, K L; Stecenko, A A (2000) Gene therapy for acute lung injury. Intensive Care Med 26 Suppl 1:S119-23

Arons, M M; Wheeler, A P; Bernard, G R et al. (1999) Effects of ibuprofen on the physiology and survival of hypothermic sepsis. Ibuprofen in Sepsis Study Group. Crit Care Med 27:699-707

Peters, M T; Brigham, K L; King, G A et al. (1999) Optimization of cationic liposome-mediated gene transfer to human bronchial epithelial cells expressing wild-type or abnormal cystic fibrosis transmembrane conductance regulator (CFTR). Exp Lung Res 25:183-97

Wheeler, A P; Bernard, G R (1999) Treating patients with severe sepsis. N Engl J Med 340:207-14

Snapper, J R; Trochtenberg, D S; Hwang, Y S et al. (1999) Effect of pulmonary edema on tracheal diameter. Respiration 66:522-7

Dupont, W D; Plummer Jr, W D (1998) Power and sample size calculations for studies involving linear regression. Control Clin Trials 19:589-601

Clark, M P; Chow, C W; Rinaldo, J E et al. (1998) Multiple domains for initiator binding proteins TFII-I and YY-1 are present in the initiator and upstream regions of the rat XDH/XO TATA-less promoter. Nucleic Acids Res 26:2813-20