Abstract

This core will provide monoclonal antibodies for several of the projects, maintain and store monoclonal antibody producing cell lines, and provide antibody purification expertise for SCOR investigators. If at a later stage of the projects, """"""""humanization"""""""" of antibodies is required, the core has the technology to sequence the variable domain genes of antibody secreting cells for use in construction of chimeric antibodies and humanization of murine monoclonal antibodies containing human heavy and light chain constant domain genes and restriction sites allowing joining of the VH or VL of murine origin transfected into murine myeloma cell lines. Antibodies will be prepared and provided for Projects 1, 3, 4, 5, and 6. Antibodies prepared to the CFTR-FLAG constructs in Project 4 may be used in Project 2 as well.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
1P50HL050160-01
Application #
3781166
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Liedtke, C M; Cody, D; Cole, T S (2001) Differential regulation of Cl- transport proteins by PKC in Calu-3 cells. Am J Physiol Lung Cell Mol Physiol 280:L739-47
Liedtke, C M; Cole, T S (2000) PKC signaling in CF/T43 cell line: regulation of NKCC1 by PKC-delta isotype. Biochim Biophys Acta 1495:24-33
Steagall, W K; Drumm, M L (1999) Stimulation of cystic fibrosis transmembrane conductance regulator-dependent short-circuit currents across DeltaF508 murine intestines. Gastroenterology 116:1379-88
Nanduri, J; Mitra, S; Andrei, C et al. (1999) An unexpected link between the secretory path and the organization of the nucleus. J Biol Chem 274:33785-9
Kelley, T J; Cotton, C U; Drumm, M L (1998) Regulation of amiloride-sensitive sodium absorption in murine airway epithelium by C-type natriuretic peptide. Am J Physiol 274:L990-6
Kelley, T J; Drumm, M L (1998) Inducible nitric oxide synthase expression is reduced in cystic fibrosis murine and human airway epithelial cells. J Clin Invest 102:1200-7
Steagall, W K; Kelley, T J; Marsick, R J et al. (1998) Type II protein kinase A regulates CFTR in airway, pancreatic, and intestinal cells. Am J Physiol 274:C819-26
Liedtke, C M; Cole, T S (1998) Antisense oligonucleotide to PKC-epsilon alters cAMP-dependent stimulation of CFTR in Calu-3 cells. Am J Physiol 275:C1357-64
Kelley, T J; Thomas, K; Milgram, L J et al. (1997) In vivo activation of the cystic fibrosis transmembrane conductance regulator mutant deltaF508 in murine nasal epithelium. Proc Natl Acad Sci U S A 94:2604-8
Kelley, T J; Al-Nakkash, L; Drumm, M L (1997) C-type natriuretic peptide increases chloride permeability in normal and cystic fibrosis airway cells. Am J Respir Cell Mol Biol 16:464-70

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