The Cell Culture Core will provide scientific and technical support to the SCOR project by helping investigators implement and interpret the results of their hematopoietic cell culture experiments. The scientific and technical support will be provided in the following ways: a) The Project Leader of the Core unit will provide a centralized scientific and technical environment in which the scientists of this SCOR would be able to discuss and design the cell biology of their experimental plans. She will also provide experimental training in cell culture procedures to investigators who feel that the experiments should be carried out in their own laboratories. b) The technical support of the Core will be: CD34+ isolation from hematopoietic tissues (cord blood, adult blood or marrow, fetal liver and fetal blood) from normal individuals or from individuals with a particular blood phenotype (for example, Kell-null using immunoaffinity chromatography (Cellpro devices). Cryopreservation and storage of such samples which cannot be processed immediately and maintaining records of the sample bank. Progenitor cell assay and liquid culture expansion of defined cell lineages (erythroid, myeloid and mast cells) under culture conditions which would allow the expansion of only one well defined differentiation lineage. The core will also establish and maintain stromal cell lines from normal bone marrow. Preparation of tested chemical and cellular reagents for primary hematopoietic cultures.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL054459-05
Application #
6302334
Study Section
Project Start
2000-02-15
Project End
2000-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2000
Total Cost
$236,465
Indirect Cost
Name
New York Blood Center
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10065
Migliaccio, Anna Rita (2018) A vicious interplay between genetic and environmental insults in the etiology of blood cancers. Exp Hematol 59:9-13
Ciaffoni, Fiorella; Cassella, Elena; Varricchio, Lilian et al. (2015) Activation of non-canonical TGF-?1 signaling indicates an autoimmune mechanism for bone marrow fibrosis in primary myelofibrosis. Blood Cells Mol Dis 54:234-41
Hricik, Todd; Federici, Giulia; Zeuner, Ann et al. (2013) Transcriptomic and phospho-proteomic analyzes of erythroblasts expanded in vitro from normal donors and from patients with polycythemia vera. Am J Hematol 88:723-9
Poletto, Valentina; Rosti, Vittorio; Villani, Laura et al. (2012) A3669G polymorphism of glucocorticoid receptor is a susceptibility allele for primary myelofibrosis and contributes to phenotypic diversity and blast transformation. Blood 120:3112-7
Huang, Cheng-Han; Ye, Mao (2010) The Rh protein family: gene evolution, membrane biology, and disease association. Cell Mol Life Sci 67:1203-18
Zhu, Xiang; Rivera, Alicia; Golub, Mari S et al. (2009) Changes in red cell ion transport, reduced intratumoral neovascularization, and some mild motor function abnormalities accompany targeted disruption of the Mouse Kell gene (Kel). Am J Hematol 84:492-8
Mutschler, Manuel; Magin, Angela S; Buerge, Martina et al. (2009) NF-E2 overexpression delays erythroid maturation and increases erythrocyte production. Br J Haematol 146:203-17
Ford, Louise; Lobo, Cheryl A; Rodriguez, Marilis et al. (2007) Differential antibody responses to Plasmodium falciparum invasion ligand proteins in individuals living in malaria-endemic areas in Brazil and Cameroon. Am J Trop Med Hyg 77:977-83
Peng, Jianbin; Redman, Colvin M; Wu, Xu et al. (2007) Insights into extensive deletions around the XK locus associated with McLeod phenotype and characterization of two novel cases. Gene 392:142-50
Hue-Roye, Kim; Lomas-Francis, Christine; Belaygorod, Larisa et al. (2007) Three new high-prevalence antigens in the Cromer blood group system. Transfusion 47:1621-9

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