Abstract

The purpose of the hematopoietic stem cell/fluorescence activated cell sorter (HSC/FACS) Core is two- fold: 1) Provide phenotypically identified hematopoietic progenitor (CD34+) and stem cells (CD34+CD38-) for both the basic and clinical projects; 2) Analyze cell populations by flow cytometry to determine expression of differentiation antigens, markers of retroviral transduction, or cell cycle analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL054850-02
Application #
5214346
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Candotti, Fabio; Shaw, Kit L; Muul, Linda et al. (2012) Gene therapy for adenosine deaminase-deficient severe combined immune deficiency: clinical comparison of retroviral vectors and treatment plans. Blood 120:3635-46
Rappeport, Joel M; O'Reilly, Richard J; Kapoor, Neena et al. (2010) Hematopoietic stem cell transplantation for severe combined immune deficiency or what the children have taught us. Immunol Allergy Clin North Am 30:17-30
Bauer, Gerhard; Dao, Mo A; Case, Scott S et al. (2008) In vivo biosafety model to assess the risk of adverse events from retroviral and lentiviral vectors. Mol Ther 16:1308-15
Dao, Mo A; Nolta, Jan A (2007) Cytokine and integrin stimulation synergize to promote higher levels of GATA-2, c-myb, and CD34 protein in primary human hematopoietic progenitors from bone marrow. Blood 109:2373-9
Engel, Barbara C; Podsakoff, Greg M; Ireland, Joanna L et al. (2007) Prolonged pancytopenia in a gene therapy patient with ADA-deficient SCID and trisomy 8 mosaicism: a case report. Blood 109:503-6
Hollis, Roger P; Nightingale, Sarah J; Wang, Xiuli et al. (2006) Stable gene transfer to human CD34(+) hematopoietic cells using the Sleeping Beauty transposon. Exp Hematol 34:1333-43
Hess, David A; Wirthlin, Louisa; Craft, Timothy P et al. (2006) Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood 107:2162-9
Montecino-Rodriguez, Encarnacion; Leathers, Hyosuk; Dorshkind, Kenneth (2006) Identification of a B-1 B cell-specified progenitor. Nat Immunol 7:293-301
Buckley, S; Barsky, L; Weinberg, K et al. (2005) In vivo inosine protects alveolar epithelial type 2 cells against hyperoxia-induced DNA damage through MAP kinase signaling. Am J Physiol Lung Cell Mol Physiol 288:L569-75
Podsakoff, Greg M; Engel, Barbara C; Carbonaro, Denise A et al. (2005) Selective survival of peripheral blood lymphocytes in children with HIV-1 following delivery of an anti-HIV gene to bone marrow CD34(+) cells. Mol Ther 12:77-86

Showing the most recent 10 out of 55 publications