Abstract

Genotypes for linkage and association were carried out in support of investigators in the current SCOR. A total of 41 separate marker systems from 21 genes were run. In all, 6,647 genotypes were obtained for this study. As new samples were arriving at the Core throughout the course of the grant, not all of the samples have been typed for all marker systems, however, an effort was made to complete typing on the new samples for key marker systems that yielded promising results in the original analysis. The Molecular Genetics core currently is completing the genotyping on the last group of a total of 214 sibling pair samples and for association studies and an additional 120 singleton samples for a total of 434 DNA samples. Linkage markers close to candidate genes as well as single nucleotide polymorphism (SNPs) within the candidate genes were run. Linkage markers were carried out using a radioactive/acrylamide detection system. SNPs were detected with either mass spectrometry, the allelic discrimination system of the Perkin-Elmer ABI Prism 7700, or restriction enzyme digestion followed by resolution on agarose gels.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
2P50HL055000-06
Application #
6415967
Study Section
Project Start
1996-02-01
Project End
2006-01-31
Budget Start
Budget End
Support Year
6
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Manosroi, Worapaka; Tan, Jia Wei; Rariy, Chevon M et al. (2017) The Association of Estrogen Receptor-? Gene Variation With Salt-Sensitive Blood Pressure. J Clin Endocrinol Metab 102:4124-4135
Gupta, Tina; Connors, Molly; Tan, Jia Wei et al. (2017) Striatin Gene Polymorphic Variants Are Associated With Salt Sensitive Blood Pressure in Normotensives and Hypertensives. Am J Hypertens 31:124-131
Tan, Jia W; Gupta, Tina; Manosroi, Worapaka et al. (2017) Dysregulated aldosterone secretion in persons of African descent with endothelin-1 gene variants. JCI Insight 2:
Chong, Cherish; Hamid, Anis; Yao, Tham et al. (2017) Regulation of aldosterone secretion by mineralocorticoid receptor-mediated signaling. J Endocrinol 232:525-534
Baudrand, Rene; Pojoga, Luminita H; Vaidya, Anand et al. (2015) Statin Use and Adrenal Aldosterone Production in Hypertensive and Diabetic Subjects. Circulation 132:1825-33
Garza, Amanda E; Rariy, Chevon M; Sun, Bei et al. (2015) Variants in striatin gene are associated with salt-sensitive blood pressure in mice and humans. Hypertension 65:211-217
Brown, Jenifer M; Williams, Jonathan S; Luther, James M et al. (2014) Human interventions to characterize novel relationships between the renin-angiotensin-aldosterone system and parathyroid hormone. Hypertension 63:273-80
Saxena, A R; Chamarthi, B; Williams, G H et al. (2014) Predictors of plasma and urinary catecholamine levels in normotensive and hypertensive men and women. J Hum Hypertens 28:292-7
Garg, Rajesh; Sun, Bei; Williams, Jonathan (2014) Effect of low salt diet on insulin resistance in salt-sensitive versus salt-resistant hypertension. Hypertension 64:1384-7
Brown, Jenifer M; Underwood, Patricia C; Ferri, Claudio et al. (2014) Aldosterone dysregulation with aging predicts renal vascular function and cardiovascular risk. Hypertension 63:1205-11

Showing the most recent 10 out of 87 publications