The purpose of Core C - Gene Expression Analysis is to provide the projects with the highlyspecialized methodology required to: (1) extract high quality RNA from human and animal samplesand perform quality control tests; (2) process RNA to produce high quality labeled cRNA forhybridization to the microarrays; (3) provide image capture and data mining software necessary foranalyzing the vast amount of gene expression data generated by microarray technology; (4)perform data analysis tailored for each individual project; and (5) to quantify mRNA levels byTaqMan real time PCR for the genes selected by the microarray screen. The Gene ExpressionAnalysis Core has extensive expertise in Affymetrix microarray data analysis using differentmethods and computational tools for data mining, particularly of samples obtained from the lungsof human volunteers by bronchoscopy/brushing, and bronchoalveolar lavage. The Core has at itsdisposal the Affymetrix GeneChip equipment, as well as state-of-the-art software packages thatare available through the Weill Cornell Institute for Computational Biomedicine. TaqMan real-timeRT-PCR for RNA quantitation is available through this Core, using high throughput 96 well AppliedBiosystems 7500 and software to design specific primers for the genes of interest, as well astechnical support and training to assist the investigators in each of the projects to carry outTaqMan analysis. The Core will devote equal resources to all five projects.
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