Abstract

The Cell and Vector Core (Core B) will provide stem and progenitor cells (human endothelial, hematopoietic, embryonic), reagents, and lentiviral vectors for investigators in all Projects, and for the Pilot and Feasibility Program. Core B will isolate rhesus and human endothelial progenitor cells (EPCs) from blood and vessels, co-culture rhesus and human EPC and hematopoietic stem cells (HSC), provide lentiviral vectors for transduction of HSC and EPC, and prepare cells for transplant for Project 1. Core B will also grow and cryopreserve renal precursors and EPCs, provide lentiviral vectors for transduction of renal precursors and EPC, and prepare cells for transplant for Project 2. For Project 3, the Core will provide human cells and lentiviral vectors, co-culture differentiated embryonic stem cells and EPC, and transduce cells and prepare for transplant (human HSC, EPC, embryonic stem cells). The Core will also provide cells and vectors to investigators funded through the Pilot and Feasibility Program.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL085036-04
Application #
7670376
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
4
Fiscal Year
2008
Total Cost
$459,667
Indirect Cost

  Institution

Name
University of California Davis
Department
Type
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Tarantal, Alice F; Berglund, Lars (2014) Obesity and lifespan health--importance of the fetal environment. Nutrients 6:1725-36
Batchelder, Cynthia A; Keyser, Jennifer L; Lee, C Chang I et al. (2013) Characterization of growth, glomerular number, and tubular proteins in the developing rhesus monkey kidney. Anat Rec (Hoboken) 296:1747-57
Hoch, Allison I; Binder, Bernard Y; Genetos, Damian C et al. (2012) Differentiation-dependent secretion of proangiogenic factors by mesenchymal stem cells. PLoS One 7:e35579
Shelley, W Chris; Leapley, Alyssa C; Huang, Lan et al. (2012) Changes in the frequency and in vivo vessel-forming ability of rhesus monkey circulating endothelial colony-forming cells across the lifespan (birth to aged). Pediatr Res 71:156-61
He, Jiawei; Decaris, Martin L; Leach, J Kent (2012) Bioceramic-mediated trophic factor secretion by mesenchymal stem cells enhances in vitro endothelial cell persistence and in vivo angiogenesis. Tissue Eng Part A 18:1520-8
Nakayama, Karina H; Batchelder, Cynthia A; Lee, Chang I et al. (2011) Renal tissue engineering with decellularized rhesus monkey kidneys: age-related differences. Tissue Eng Part A 17:2891-901
Ivanova, Larissa; Hiatt, Michael J; Yoder, Mervin C et al. (2010) Ontogeny of CD24 in the human kidney. Kidney Int 77:1123-31
Nakayama, Karina H; Batchelder, Cynthia A; Lee, Chang I et al. (2010) Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering. Tissue Eng Part A 16:2207-16
Lee, C Chang I; Christensen, Jared E; Yoder, Mervin C et al. (2010) Clonal analysis and hierarchy of human bone marrow mesenchymal stem and progenitor cells. Exp Hematol 38:46-54
Trnka, Peter; Hiatt, Michael J; Ivanova, Larissa et al. (2010) Phenotypic transition of the collecting duct epithelium in congenital urinary tract obstruction. J Biomed Biotechnol 2010:696034

Showing the most recent 10 out of 23 publications