In order to effectively make use of the technology and labor intensive procedures of viral genetics, we have established a virology core. Viral mutagenesis is currently one of the most powerful technologies available to analyze viral genetics, and to test hypothesis on herpes viral latency and reactivation. The burden to our outstanding geneticists, molecular biologists, and other scientists who must expend large amounts of resources engineering and growing viral reagents, has limited our progress for years. The creation of a virology core will free the virologists and non-virologists to pursue their scientific goal in understanding the mechanism of HSV latency and reactivation. The core facility will provide expertise in engineering, selecting, and growing viruses. It will, with assistance of the administrative core, maintain an archive of plasmid and viral constructs, which will be available to the program investigators, and for shipment to other laboratories. It will, when requested, assist individual projects in infecting animals with viral stocks (using animal protocol from individual projects).

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Specialized Center (P50)
Project #
2P50NS033768-15A2
Application #
6553197
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
1986-08-01
Project End
2006-08-31
Budget Start
Budget End
Support Year
15
Fiscal Year
2001
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Sanders, Iryna; Boyer, Mark; Fraser, Nigel W (2015) Early nucleosome deposition on, and replication of, HSV DNA requires cell factor PCNA. J Neurovirol 21:358-69
Oh, Jaewook; Sanders, Iryna F; Chen, Eric Z et al. (2015) Genome wide nucleosome mapping for HSV-1 shows nucleosomes are deposited at preferred positions during lytic infection. PLoS One 10:e0117471
Brinkman, Kerry K; Mishra, Prakhar; Fraser, Nigel W (2013) The half-life of the HSV-1 1.5-kb LAT intron is similar to the half-life of the 2.0-kb LAT intron. J Neurovirol 19:102-8
Volcy, Ketna; Fraser, Nigel W (2013) DNA damage promotes herpes simplex virus-1 protein expression in a neuroblastoma cell line. J Neurovirol 19:57-64
Millhouse, Scott; Wang, Xiaohe; Fraser, Nigel W et al. (2012) Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner. J Neurovirol 18:231-43
Oh, Jaewook; Ruskoski, Nicholas; Fraser, Nigel W (2012) Chromatin assembly on herpes simplex virus 1 DNA early during a lytic infection is Asf1a dependent. J Virol 86:12313-21
Jiang, Xianzhi; Chentoufi, Aziz Alami; Hsiang, Chinhui et al. (2011) The herpes simplex virus type 1 latency-associated transcript can protect neuron-derived C1300 and Neuro2A cells from granzyme B-induced apoptosis and CD8 T-cell killing. J Virol 85:2325-32
Millhouse, Scott; Su, Ying-Hsiu; Zhang, Xianchao et al. (2010) Evidence that herpes simplex virus DNA derived from quiescently infected cells in vitro, and latently infected cells in vivo, is physically damaged. J Neurovirol 16:384-98
Smith, Sheryl T; Wickramasinghe, Priyankara; Olson, Andrew et al. (2009) Genome wide ChIP-chip analyses reveal important roles for CTCF in Drosophila genome organization. Dev Biol 328:518-28