DC-memory T cell mixtures derived from the body surfaces support SIV replication. Both skin-and blood-derived DC populations promote virus replication in the presence of CD4+ T cells. We have been investigating whether DC subsets interact differently with naove versus memory T cells. Immature and mature blood DCs exposed to virus were mixed with memory or naove CD4+ T cells. Combinations of mature DCs with either naove or memory T cells support virus replication. In the presence of immature DCs and memory T cells virus replication is comparable to that seen in the presence of mature DCs. In contrast, virus production in the immature DC-naove T cell cultures exhibits a minimum 2-4 day delay. Therefore, the environment of DC-memory T cells, much like that at the mucosal surfaces of the body, is very permissive to SIV replication. Two animals were s.c. injected with in vitro SIV-infected autologous immature DCs. Both animals were coculture positive within 1 week, with peak viremia within 2 weeks. Although the viral levels in both animals declined after the initial peak, this was short-lived for one animal, as the viral load began to increase again. This animal did not develop any anti-SIV Ab and recently had to be euthanized [19 weeks post injection]. Blood and tissue samples have been collected and will be analyzed. We recently injected another two animals with DCs as well as two control animals with SIV-infected CD4+ T cells. All animals receiving infected DCs have become rapidly infected and 1 of the 2 animals that received infected T cells is coculture positive. We are awaiting bDNA data. FUNDING NIH (R01 AI40877) PUBLICATIONS Ignatius, R., F. Isdell, U. O?Doherty and M. Pope. Dendritic cells from skin and blood of macaques both promote SIV replication with T cells from different anatomical sites. Journal of Medical Primatology, 27:121-128, 1998. Hu, J., M. Pope, C. Brown, U. O?Doherty and C.J. Miller. Immunophenotypic characterization of simian immunodeficiency virus-infected dendritic cells in cervix, vagina and draining lymph nodes of rhesus monkeys. Laboratory Investigator, 78:435-451, 1998.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000164-38
Application #
6116181
Study Section
Project Start
1999-05-01
Project End
2000-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
38
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Tulane University
Department
Type
DUNS #
City
New Orleans
State
LA
Country
United States
Zip Code
70118
Mahalingam, Ravi; Kaufer, Benedikt B; Ouwendijk, Werner J D et al. (2018) Attenuation of Simian Varicella Virus Infection by Enhanced Green Fluorescent Protein in Rhesus Macaques. J Virol 92:
Kumar, Vinay; Mansfield, Joshua; Fan, Rong et al. (2018) miR-130a and miR-212 Disrupt the Intestinal Epithelial Barrier through Modulation of PPAR? and Occludin Expression in Chronic Simian Immunodeficiency Virus-Infected Rhesus Macaques. J Immunol 200:2677-2689
Parthasarathy, Geetha; Philipp, Mario T (2018) Intracellular TLR7 is activated in human oligodendrocytes in response to Borrelia burgdorferi exposure. Neurosci Lett 671:38-42
McNamara, Ryan P; Costantini, Lindsey M; Myers, T Alix et al. (2018) Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses. MBio 9:
Calenda, Giulia; Villegas, Guillermo; Barnable, Patrick et al. (2017) MZC Gel Inhibits SHIV-RT and HSV-2 in Macaque Vaginal Mucosa and SHIV-RT in Rectal Mucosa. J Acquir Immune Defic Syndr 74:e67-e74
Datta, Dibyadyuti; Bansal, Geetha P; Grasperge, Brooke et al. (2017) Comparative functional potency of DNA vaccines encoding Plasmodium falciparum transmission blocking target antigens Pfs48/45 and Pfs25 administered alone or in combination by in vivo electroporation in rhesus macaques. Vaccine 35:7049-7056
Yi, Fei; Guo, Jia; Dabbagh, Deemah et al. (2017) Discovery of Novel Small-Molecule Inhibitors of LIM Domain Kinase for Inhibiting HIV-1. J Virol 91:
Jorgensen, Matthew J; Lambert, Kelsey R; Breaux, Sarah D et al. (2017) Pair housing of Vervets/African Green Monkeys for biomedical research. Am J Primatol 79:1-10
Ramesh, Geeta; Martinez, Alejandra N; Martin, Dale S et al. (2017) Effects of dexamethasone and meloxicam on Borrelia burgdorferi-induced inflammation in glial and neuronal cells of the central nervous system. J Neuroinflammation 14:28
Parthasarathy, Geetha; Philipp, Mario T (2017) Receptor tyrosine kinases play a significant role in human oligodendrocyte inflammation and cell death associated with the Lyme disease bacterium Borrelia burgdorferi. J Neuroinflammation 14:110

Showing the most recent 10 out of 352 publications