This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Regarding AIDSThe Flow Cytometry Core Laboratory instrumentation includes 2 FACSCaliburs, capable of 4-parameter acquisitions of data, 1 LSRII capable of running 12 different flourochrome, and 1 FACSAria high-speed cell sorter capable of multi-parameter acquisition of up to 13 flourochomes. The FACSAria can also separate up to 4 subpopulations of cells. In addition to acquiring a 4-color data, the FACSCaliburs performed other assays such as cell proliferation, using carboxy-flourescein diacetate succinimidyl ester (CFSE), Cytometric Bead Array (CBA) for cytokine detection in plasma or supernatants, and cell ploidy determinations. The FACSAria has been extensively used for 6- to 8-color acquisition and 2- to 4-way high speed sorting. This contributes to a total workload increase for the flow core lab of 22.4%. The needs of individual investigators varied. Some investigators utilized the comprehensive services offered by the core lab: from monoclonal antibody selection through data acquisition. The majority of investigators processed their own samples in preparation fro flow cytometry acquisition. The core lab provides limited data analysis; most investigators perform their own data analysis. Raw data files are distributed to the various investigators via a secure Tulane National Primate Research Center server. We experienced an increase in total workload as evidenced by the 34,861 FACSCalibur samples and the 6,976 FACSAria samples processed. This increase is due to an upsurge in the numbers of samples categorized as 'acquisition alone' (FACSCalibur) and the use of the FACSAria for multi-parameter data acquisition. The sorting capabilities of the FACSAria/LSRII played an important role in the research efforts of several in-house investigators.
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