This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.In order to better understand genes regulated by RpoS, the alternative Sigma factor in the Lyme disease spirochete, we are analyzing the promoter regions of genes that are controlled by RpoS. We mutated the promoter regions of the ospC gene to identify sequence elements that confer RpoS specificity. Preliminary results indicate that the specificity of promoter recognition lies in the -10 element. We are currently confirming this by including other mutations in the ospC promoter as well as the promoter regions of at least two other genes. The results from this study will help us identify other RpoS-controlled genes in these organisms.
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