This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To provide core, campus and non-host investigators with sophisticated and specialized genetics resources and expertise.? ? Progress and concerns? ? In 2006, the Genetics Service established a high-throughput DNA sequencing facility. From May 1, 2006 to December ? 31, 2006, the sequencing facility processed more than 30,000 DNA sequences for 22 users. Sequence quality was high, ? with an average read length of more than 670 bases in December, 2006. Responding to the popularity of the service in ? 2006, we recently upgraded the sequencing hardware to double throughput and capacity. The Genetics Service also ? provides increasingly sophisticated MHC genotyping of WNPRC macaques. The tools developed for these analyses are ? now being adapted by outside investigators with WNPRC assistance.? ? Allocation of resource access? ? The Genetics Service sequencing facility was designed as an economical and rapid alternative to existing DNA sequencing ? services for nonhuman primate researchers with medium-to-high throughput needs. Current clients are drawn largely ? from WNPRC AIDS laboratories that perform extensive SIV and immunogenetics research.? ? Dissemination? ? We request that projects utilizing Genetics Service acknowledge the service in manuscripts and presentations. Dr. ? O'Connor, the PI for Genetics Services, lectures regularly on NHP genetics.? ? Training? ? All 22 users of the sequencing service received training in the submission and retrieval of data during 2006. A subset of ? these users obtained advanced training in the analysis of sequence data from Genetics Services staff. Additionally, ? methods for MHC genotyping macaques using microsatellite markers were provided to laboratories in the USA, UK, and ? Australia.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
3P51RR000167-46S2
Application #
7637115
Study Section
Special Emphasis Panel (ZRR1-CM-9 (01))
Project Start
2007-05-10
Project End
2008-04-30
Budget Start
2007-05-10
Budget End
2008-04-30
Support Year
46
Fiscal Year
2007
Total Cost
$536,055
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Kang, HyunJun; Mesquitta, Walatta-Tseyon; Jung, Ho Sun et al. (2018) GATA2 Is Dispensable for Specification of Hemogenic Endothelium but Promotes Endothelial-to-Hematopoietic Transition. Stem Cell Reports 11:197-211
Rhoads, Timothy W; Burhans, Maggie S; Chen, Vincent B et al. (2018) Caloric Restriction Engages Hepatic RNA Processing Mechanisms in Rhesus Monkeys. Cell Metab 27:677-688.e5
Ellis-Connell, Amy L; Balgeman, Alexis J; Zarbock, Katie R et al. (2018) ALT-803 Transiently Reduces Simian Immunodeficiency Virus Replication in the Absence of Antiretroviral Treatment. J Virol 92:
Park, Mi Ae; Jung, Ho Sun; Slukvin, Igor (2018) Genetic Engineering of Human Pluripotent Stem Cells Using PiggyBac Transposon System. Curr Protoc Stem Cell Biol 47:e63
Ellis, Amy; Balgeman, Alexis; Rodgers, Mark et al. (2017) Characterization of T Cells Specific for CFP-10 and ESAT-6 in Mycobacterium tuberculosis-Infected Mauritian Cynomolgus Macaques. Infect Immun 85:
Rodrigues, Michelle A (2017) Female Spider Monkeys (Ateles geoffroyi) Cope with Anthropogenic Disturbance Through Fission-Fusion Dynamics. Int J Primatol 38:838-855
Buechler, Connor R; Bailey, Adam L; Lauck, Michael et al. (2017) Genome Sequence of a Novel Kunsagivirus (Picornaviridae: Kunsagivirus) from a Wild Baboon (Papio cynocephalus). Genome Announc 5:
Wu, Hong; Whritenour, Jessica; Sanford, Jonathan C et al. (2017) Identification of MHC Haplotypes Associated with Drug-induced Hypersensitivity Reactions in Cynomolgus Monkeys. Toxicol Pathol 45:127-133
Shackman, A J; Fox, A S; Oler, J A et al. (2017) Heightened extended amygdala metabolism following threat characterizes the early phenotypic risk to develop anxiety-related psychopathology. Mol Psychiatry 22:724-732
Kalin, Ned H (2017) Mechanisms underlying the early risk to develop anxiety and depression: A translational approach. Eur Neuropsychopharmacol 27:543-553

Showing the most recent 10 out of 528 publications