This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To generate iPS cells from SLE patients and use them as a scalable source of autologous primitive hematopoietic cells for transplantation, as well as regulatory dendritic (DC) and T cells for immunotherapy in Lupus patients. Induced pluripotent stem (iPS) cells are stem cells artificially derived from somatic fibroblasts through insertion of certain genes critical for the maintenance of pluripotency of human embryonic stem (hES) cells. It has been shown that these cells behave similar to hES cells, i.e. capable of self-renewal and large-scale expansion and differentiation toward all three germ layers. Our idea is that iPS cells can be generated from SLE patients and used as a scalable source of autologous primitive hematopoietic cells for transplantation, as well as regulatory dendritic (DC) and T cells for immunotherapy. Because iPS cells are similar to hES cells, they can be sustainably expanded and genetically altered in a highly controlled way that is not yet possible with more committed HSCs. Thus, iPS cells could provide important advantages over somatic HSCs as target cells for stem cell based therapy of SLE. In this application we are aiming to identify cell subpopulations and conditions essential for generation of engraftable hematopoietic cells, T cells and DC from iPS cells. This research used WNPRC Stem Cell Resources.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000167-49
Application #
8173133
Study Section
Special Emphasis Panel (ZRR1-CM-8 (01))
Project Start
2010-05-01
Project End
2011-04-30
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
49
Fiscal Year
2010
Total Cost
$41,308
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Other Domestic Higher Education
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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