This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.NK cells in rhesus macaques have been variably defined as CD3-CD16+ or CD3-CD8+, although only limited efforts have been made to rigorously validate these definitions. To better understand the role of NK cells in macaque disease models, we undertook a multiparameter analysis of macaque NK cells employing four color flow cytometry and a panel of lineage-specific and non-lineage specific lymphocyte markers. Using this approach, we recently identified two distinct populations of candidate NK cells: a major CD8brightCD16+ population and a minor CD8brightCD16- population. In addition, a CD56+ subset of cells within the minor rhesus NK population was identified that expressed chemokine and lymph node homing receptors similar to those expressed by the CD56bright NK cell population identified in humans. This work has now been extended using 8 color flow cytometry and has verified the existence of this different populations of NK cells. In addition, we have developed a functional flow cytometric assay for the ability of NK cells to degranulate in response to stimulation with target cells. These improved immunophenotypic definitions of macaque NK cells should facilitate future analysis of innate immune responses in rhesus macaques and the role of NK cells in AIDS pathogenesis in SIV-infected macaques.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
2P51RR000168-47
Application #
7715453
Study Section
Special Emphasis Panel (ZRR1-CM-8 (01))
Project Start
2008-06-05
Project End
2009-04-30
Budget Start
2008-06-05
Budget End
2009-04-30
Support Year
47
Fiscal Year
2008
Total Cost
$129,768
Indirect Cost
Name
Harvard University
Department
Veterinary Sciences
Type
Schools of Medicine
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
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