This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The objectives of these studies are to characterize and quantify nonviral gene delivery of nucleic acids complexed with cationic lipids to the central nervous system (CNS) of the monkey, including the extent of expression of candidate neuroprotective genes. These experiments will lead to the development of DNA and mRNA as gene medicines for transient expression in a variety of clinical applications, such as pre-operatively to protect the brain. As a first step towards clinical use, it is essential to ensure that the nonviral delivery techniques are well-tolerated. It is equally important to examine the duration and intensity of expression, and to determine what other tissues, if any, demonstrate expression after delivery to the cerebrospinal fluid in the CNS.
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