The DNA Sequencing Core will provide UM-MAC investigators access to inexpensive, automated DNA sequencing technology on a recharge basis. Core operation is substantially more effective than individual manual sequencing, since the Core to operates sequencers and purchases reagents in bulk. Since more than 30 UM-MAC members have identified themselves as current or future users of this core, we are proposing to include support for the DNA Sequencing Core in the next UM-MAC funding cycle. The Sequencing Core occupies 750 square feet of laboratory space and employs three sequencing technicians to operate three ABI model 373 DNA Sequencers (one of which has been upgraded to a 'Stretch' Model). A fourth sequencer, an ABI Model 377, is on order. Samples are typically processed in 2 days or less, yielding 350-400 nucleotides of DNA sequence data at better than 99% accuracy. Sample submission and tracking is computerized, and data are automatically returned to the investigator by electronic mail. The basic cost for this service is $25 per sample (one template, one primer). UM-MAC members would be given preferred discounts (50% below normal recharge rate) for use of the core. Funding for the DNA Sequencing Core is derived primarily from user fees, with some additional support from institutional sources (Office of the Vice President for Research, Office of the Provost, Dean of the School of Medicine) as well as a variety of research center grants. Center grants providing support for the Core are given a 50% discount in the recharge rate. This decreased price encourages researchers to make use of the sequencing service, saving them valuable time and improving the cost- effectiveness of their use of grant funds.

Project Start
1998-01-15
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
20
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Davis, Shannon W; Keisler, Jessica L; Pérez-Millán, María I et al. (2016) All Hormone-Producing Cell Types of the Pituitary Intermediate and Anterior Lobes Derive From Prop1-Expressing Progenitors. Endocrinology 157:1385-96
Gorelik, Gabriela; Sawalha, Amr H; Patel, Dipak et al. (2015) T cell PKC? kinase inactivation induces lupus-like autoimmunity in mice. Clin Immunol 158:193-203
Rillamas-Sun, Eileen; Harlow, Siobán D; Randolph Jr, John F (2014) Grandmothers' smoking in pregnancy and grandchildren's birth weight: comparisons by grandmother birth cohort. Matern Child Health J 18:1691-8
O'Leary, Erin E; Mazurkiewicz-Muñoz, Anna M; Argetsinger, Lawrence S et al. (2013) Identification of steroid-sensitive gene-1/Ccdc80 as a JAK2-binding protein. Mol Endocrinol 27:619-34
Sugg, Kristoffer B; Rosenthal, Andrew H; Ozaki, Wayne et al. (2013) Quantitative comparison of volume maintenance between inlay and onlay bone grafts in the craniofacial skeleton. Plast Reconstr Surg 131:1014-21
Perez-Millan, Maria Ines; Zeidler, Michael G; Saunders, Thomas L et al. (2013) Efficient, specific, developmentally appropriate cre-mediated recombination in anterior pituitary gonadotropes and thyrotropes. Genesis 51:785-92
Fang, Qing; Giordimaina, Alicia M; Dolan, David F et al. (2012) Genetic background of Prop1(df) mutants provides remarkable protection against hypothyroidism-induced hearing impairment. J Assoc Res Otolaryngol 13:173-184
Tao, Jiayi; Zhu, Min; Wang, He et al. (2012) SEC23B is required for the maintenance of murine professional secretory tissues. Proc Natl Acad Sci U S A 109:E2001-9
Rillamas-Sun, Eileen; Sowers, MaryFran R; Harlow, Sioban D et al. (2012) The relationship of birth weight with longitudinal changes in body composition in adult women. Obesity (Silver Spring) 20:463-5
Castinetti, F; Brinkmeier, M L; Gordon, D F et al. (2011) PITX2 AND PITX1 regulate thyrotroph function and response to hypothyroidism. Mol Endocrinol 25:1950-60

Showing the most recent 10 out of 373 publications