The immunohistochemical identification of antigens on tissue sections has become an integral and valuable part of modern histopathology. Antigens are localized either by immunofluorescent techniques or by enzyme- substrate methods on embedded or frozen materials. Although these methods are routine for soft tissue samples, immunohistochemical examination of osteocartilaginous tissues is made difficult by the material properties of bone and cartilage. Special techniques are required to process nondecalcified bone for immunohistochemistry such as the use of water soluble, cold polymerizing plastics or the use of special adhesives, supports, and slides after thin sectioning of frozen material on a motorized cryostat. With these techniques one can identify antigens present on the surface of cells, whether in inflammatory infiltrates, in cartilage, bone, synovium, or any other tissue; and noncellular antigens present within the matrix. The objective of the Immunohistochemistry Core of the northeast ohio multipurpose Arthritis Center is to continue to function as a shared resource and centralized facility for the production of monoclonal antibodies relevant to musculoskeletal research and for the processing, embedding, cutting, and immunohistochemical staining of bone, cartilage, and joint-related samples generated as part of on-going research activities of center personnel. The centralization of the immunohistochemistry laboratory provides economy of supplies, equipment, and effort; offers new technology to investigators not currently using these techniques; and promotes interactions among the various investigators in the NEOMAC.

Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
1996
Total Cost
Indirect Cost
Miwa, Hazuki E; Gerken, Thomas A; Huynh, Tru D et al. (2009) Conserved sequence in the aggrecan interglobular domain modulates cleavage by ADAMTS-4 and ADAMTS-5. Biochim Biophys Acta 1790:161-72
Atif, U; Philip, A; Aponte, J et al. (2008) Absence of association of asporin polymorphisms and osteoarthritis susceptibility in US Caucasians. Osteoarthritis Cartilage 16:1174-7
Kraus, V B; Jordan, J M; Doherty, M et al. (2007) The Genetics of Generalized Osteoarthritis (GOGO) study: study design and evaluation of osteoarthritis phenotypes. Osteoarthritis Cartilage 15:120-7
Miwa, Hazuki E; Gerken, Thomas A; Huynh, Tru D et al. (2006) Mammalian expression of full-length bovine aggrecan and link protein: formation of recombinant proteoglycan aggregates and analysis of proteolytic cleavage by ADAMTS-4 and MMP-13. Biochim Biophys Acta 1760:472-86
Holderbaum, D; Malvitz, T; Ciesielski, C J et al. (2005) A newly described hereditary cartilage debonding syndrome. Arthritis Rheum 52:3300-4
Denko, Charles W; Malemud, Charles J (2005) Role of the growth hormone/insulin-like growth factor-1 paracrine axis in rheumatic diseases. Semin Arthritis Rheum 35:24-34
Ievers-Landis, Carolyn E; Burant, Christopher; Drotar, Dennis et al. (2005) A randomized controlled trial for the primary prevention of osteoporosis among preadolescent girl scouts: 1-year outcomes of a behavioral program. J Pediatr Psychol 30:155-65
Vashishth, D (2005) Collagen glycation and its role in fracture properties of bone. J Musculoskelet Neuronal Interact 5:316
Denko, Charles W; Malemud, Charles J (2005) Serum growth hormone and insulin but not insulin-like growth factor-1 levels are elevated in patients with fibromyalgia syndrome. Rheumatol Int 25:146-51
Denko, Charles W; Malemud, Charles J (2004) Age-related changes in serum growth hormone, insulin-like growth factor-1 and somatostatin in system lupus erythematosus. BMC Musculoskelet Disord 5:37

Showing the most recent 10 out of 92 publications