Abstract

The Tissue Analysis Core will provide technical support in two major areas to projects associated with the Comprehensive Oral Health Research Center of Discovery. The first area involves tissue preparation and localization of molecules of interest, including plastic or paraffin embedding and sectioning of samples, freezing and cryostat sectioning of samples, in situ hybridization to localize mRNA, and immunocytochemistry to localize proteins. The second area involve biochemical tests of tissue extracts, or body fluids, including blood, saliva, and crevicular fluid. Molecules of interest include cytokines, carbohydrate epitopes, antibodies, and nicotine byproducts. The technical staff will be expected to keep records of all same preparation procedures. The technical staff will be expected to keep records of all sample procedures. The technical staff will also be expected to train designated members of participating laboratories in the above technologies, so that specialized or rush products can be completed in a timely manner, and so that the core lab and its resources are used beyond the typical workday. Projects 1,2,4,5,6,8,11 will be particularly heavy users of this core.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Comprehensive Center (P60)
Project #
5P60DE013058-02
Application #
6349076
Study Section
Project Start
1999-08-01
Project End
2004-07-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2000
Total Cost
$135,613
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Bronckers, Antonius L J J; Lyaruu, Don M; Guo, Jing et al. (2015) Composition of mineralizing incisor enamel in cystic fibrosis transmembrane conductance regulator-deficient mice. Eur J Oral Sci 123:9-16
Gansky, Stuart A; Cheng, Nancy F; Koch, Gary G (2011) Dose-Weighted Adjusted Mantel-Haenszel Tests for Numeric Scaled Strata in a Randomized Trial. Stat Biopharm Res 3:266-275
Walsh, Margaret M; Langer, Timothy J; Kavanagh, Niall et al. (2010) Smokeless tobacco cessation cluster randomized trial with rural high school males: intervention interaction with baseline smoking. Nicotine Tob Res 12:543-50
Gansky, Stuart A; Ellison, James A; Kavanagh, Catherine et al. (2009) Patterns and correlates of spit tobacco use among high school males in rural California. J Public Health Dent 69:116-24
Kim, Jae-Beom; Leucht, Philipp; Luppen, Cynthia A et al. (2007) Reconciling the roles of FAK in osteoblast differentiation, osteoclast remodeling, and bone regeneration. Bone 41:39-51
Saeki, Kuniko; Hilton, Joan F; Alliston, Tamara et al. (2007) Elevated TGF-beta2 signaling in dentin results in sex related enamel defects. Arch Oral Biol 52:814-21
Six, N; Septier, D; Chaussain-Miller, C et al. (2007) Dentonin, a MEPE fragment, initiates pulp-healing response to injury. J Dent Res 86:780-5
Weintraub, J A; Ramos-Gomez, F; Jue, B et al. (2006) Fluoride varnish efficacy in preventing early childhood caries. J Dent Res 85:172-6
Hsieh, Nancy Kwon; Herzig, Karen; Gansky, Stuart A et al. (2006) Changing dentists' knowledge, attitudes and behavior regarding domestic violence through an interactive multimedia tutorial. J Am Dent Assoc 137:596-603
Colnot, Celine (2005) Cellular and molecular interactions regulating skeletogenesis. J Cell Biochem 95:688-97

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