Abstract

PI G. Lutty will investigate the effect of the cytokines IL-1 and TNFalpha which up-regulate adhesion molecules on retention of human sickle erythrocytes in the retinal microvasculature in a rat model in which only SS4 cells (dense SS cells) are retained under normal conditions. A second hypothesis will be investigated that transgenic mice with higher levels of expression of human alpha and BetaS- globin genes have an earlier onset and more severe retinopathy and that the onset of vaso-occlusion occurs more rapidly if the animals receive LPS, which may stimulate up-regulation of adhesion molecules by mechanisms similar to those observed in the rat model.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Comprehensive Center (P60)
Project #
5P60HL038655-12
Application #
6109882
Study Section
Project Start
1999-04-01
Project End
2000-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
12
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Olivier, Emmanuel N; Rybicki, Anne C; Bouhassira, Eric E (2006) Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells 24:1914-22
Srinivasulu, Sonati; Perumalsamy, Krishnaveni; Upadhya, Rajendra et al. (2006) Pair-wise interactions of polymerization inhibitory contact site mutations of hemoglobin-S. Protein J 25:503-16
Oh, Il-Hoan; Fabry, Mary E; Humphries, R Keith et al. (2004) Expression of an anti-sickling beta-globin in human erythroblasts derived from retrovirally transduced primitive normal and sickle cell disease hematopoietic cells. Exp Hematol 32:461-9
Kaul, Dhananjay K; Liu, Xiao-du; Chang, Hee-Yoon et al. (2004) Effect of fetal hemoglobin on microvascular regulation in sickle transgenic-knockout mice. J Clin Invest 114:1136-45
Kaul, Dhananjay K; Fabry, Mary E (2004) In vivo studies of sickle red blood cells. Microcirculation 11:153-65
Romero, Jose R; Suzuka, Sandra M; Nagel, Ronald L et al. (2004) Expression of HbC and HbS, but not HbA, results in activation of K-Cl cotransport activity in transgenic mouse red cells. Blood 103:2384-90
Wang, Jian-Ying; Drlica, Karl (2003) Modeling hybridization kinetics. Math Biosci 183:37-47
Alami, Raouf; Fan, Yuhong; Pack, Stephanie et al. (2003) Mammalian linker-histone subtypes differentially affect gene expression in vivo. Proc Natl Acad Sci U S A 100:5920-5
Eszterhas, Susan K; Bouhassira, Eric E; Martin, David I K et al. (2002) Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position. Mol Cell Biol 22:469-79
Dewan, John C; Feeling-Taylor, Angela; Puius, Yoram A et al. (2002) Structure of mutant human carbonmonoxyhemoglobin C (betaE6K) at 2.0 A resolution. Acta Crystallogr D Biol Crystallogr 58:2038-42

Showing the most recent 10 out of 143 publications