Abstract

This core will have three functions: 1) Interface the Program with the well established and highly successful, Transgenic and Gene Targeting Facility available at the Albert Einstein College of Medicine. In this facility transgenic mice will be generated with DNA constructs supplied by Core B. This function will be mainly to provide assistance to investigators in the labeling, identification and record keeping of the founders and their future generations. 2) Identify founders expressing the desired hemoglobins or red cell transporters, characterize them and provide assistance with breeding strategies of the founders. These include, breeding them to the transgenic mice expressing alphaH BetaS, the alpha-knockout, the Beta-knockouts, or to other transgenic and knockout lines available. 3) Breed transgenic mice which will be identified and characterized for use in Project 1 (Two animal models of organ damage in sickle cell disease); Project 2 (Transgenic expressing HbC and HbS and HbC simultaneously; Project 6 (Transgenic mice expression human K:C1 co-transport).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Comprehensive Center (P60)
Project #
3P60HL038655-15S1
Application #
6646659
Study Section
Project Start
2002-05-20
Project End
2003-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
15
Fiscal Year
2002
Total Cost
$175,153
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Type
DUNS #
009095365
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Olivier, Emmanuel N; Rybicki, Anne C; Bouhassira, Eric E (2006) Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells 24:1914-22
Srinivasulu, Sonati; Perumalsamy, Krishnaveni; Upadhya, Rajendra et al. (2006) Pair-wise interactions of polymerization inhibitory contact site mutations of hemoglobin-S. Protein J 25:503-16
Oh, Il-Hoan; Fabry, Mary E; Humphries, R Keith et al. (2004) Expression of an anti-sickling beta-globin in human erythroblasts derived from retrovirally transduced primitive normal and sickle cell disease hematopoietic cells. Exp Hematol 32:461-9
Kaul, Dhananjay K; Liu, Xiao-du; Chang, Hee-Yoon et al. (2004) Effect of fetal hemoglobin on microvascular regulation in sickle transgenic-knockout mice. J Clin Invest 114:1136-45
Kaul, Dhananjay K; Fabry, Mary E (2004) In vivo studies of sickle red blood cells. Microcirculation 11:153-65
Romero, Jose R; Suzuka, Sandra M; Nagel, Ronald L et al. (2004) Expression of HbC and HbS, but not HbA, results in activation of K-Cl cotransport activity in transgenic mouse red cells. Blood 103:2384-90
Wang, Jian-Ying; Drlica, Karl (2003) Modeling hybridization kinetics. Math Biosci 183:37-47
Alami, Raouf; Fan, Yuhong; Pack, Stephanie et al. (2003) Mammalian linker-histone subtypes differentially affect gene expression in vivo. Proc Natl Acad Sci U S A 100:5920-5
Eszterhas, Susan K; Bouhassira, Eric E; Martin, David I K et al. (2002) Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position. Mol Cell Biol 22:469-79
Dewan, John C; Feeling-Taylor, Angela; Puius, Yoram A et al. (2002) Structure of mutant human carbonmonoxyhemoglobin C (betaE6K) at 2.0 A resolution. Acta Crystallogr D Biol Crystallogr 58:2038-42

Showing the most recent 10 out of 143 publications