Abstract

One of the objectives of this project is to investigate the ultrastructure of the crustacean stretch receptor organ in relation to its function. We plan to investigate stretch-induced structural changes in the dendrities of the sensory neuron, where the generator potential occurs, by using ultraquick freezing and freeze-substitution methods. In addition, the membrane fine structure of the inhibitory synapses on the receptor neuron will be studied by using the freeze-fracture method. The other objective of this project is to investigate the ultrastructural and electrophysiological properties of newly-formed neuromuscular junctions in tissue culture. Also we will study the development of extrajunctional acetylcholine receptors in cultured muscle cells by using microelectrophysiological and freeze-fracture methods.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Project (R01)
Project #
5R01AG006093-14
Application #
3116889
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1985-08-01
Project End
1990-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
14
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

Nakajima, Yasuko; Nakajima, Shigehiro (2010) Measurement of orexin (hypocretin) and substance P effects on constitutively active inward rectifier K(+) channels in brain neurons. Methods Enzymol 484:613-30
Zhao, Qi; Albsoul-Younes, Abla M; Zhao, Peng et al. (2006) Dominant negative effects of a Gbeta mutant on G-protein coupled inward rectifier K+ channel. FEBS Lett 580:3879-82
Koike-Tani, Maki; Collins, John M; Kawano, Takeharu et al. (2005) Signal transduction pathway for the substance P-induced inhibition of rat Kir3 (GIRK) channel. J Physiol 564:489-500
Tesmer, Valerie M; Kawano, Takeharu; Shankaranarayanan, Aruna et al. (2005) Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex. Science 310:1686-90
Kawano, Takeharu; Zhao, Peng; Nakajima, Shigehiro et al. (2004) Single-cell RT-PCR analysis of GIRK channels expressed in rat locus coeruleus and nucleus basalis neurons. Neurosci Lett 358:63-7
Zhao, Qi; Kawano, Takeharu; Nakata, Hiroko et al. (2003) Interaction of G protein beta subunit with inward rectifier K(+) channel Kir3. Mol Pharmacol 64:1085-91
Hoang, Q V; Bajic, D; Yanagisawa, M et al. (2003) Effects of orexin (hypocretin) on GIRK channels. J Neurophysiol 90:693-702
Stanfield, Peter R; Nakajima, Shigehiro; Nakajima, Yasuko (2002) Constitutively active and G-protein coupled inward rectifier K+ channels: Kir2.0 and Kir3.0. Rev Physiol Biochem Pharmacol 145:47-179
Bajic, D; Koike, M; Albsoul-Younes, A M et al. (2002) Two different inward rectifier K+ channels are effectors for transmitter-induced slow excitation in brain neurons. Proc Natl Acad Sci U S A 99:14494-9
Albsoul-Younes, A M; Sternweis, P M; Zhao, P et al. (2001) Interaction sites of the G protein beta subunit with brain G protein-coupled inward rectifier K+ channel. J Biol Chem 276:12712-7

Showing the most recent 10 out of 35 publications