Abstract

We shall pursue the following objectives: 1. Add to our collection of 52 pseudorabies (Pr) virus ts mutants, virus mutants isolated after mutagenesis of specific regions of the genome. We shall construct a complete genetic and physical map of the Pr virus genome. 2. Study in detail the interactions of viral DNA molecules within the infected cells in an attempt to gain an understanding of the processes leading to the two isomeric orientations of the short unique region of the genome. 3. Study the pathways leading to the cleavage of the concatemeric viral DNA and the assembly of the viral nucleocapsids using ts mutants defective in these functions. 4. Study the transcription and processing of immediate-early (IE) RNA. The nature of the IE proteins and their functions in terms of binding to specific regions of the genome or modifying the activity of the cellular DNA-dependent RNA polymerase II will also be studied.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI010947-14
Application #
3124852
Study Section
(SSS)
Project Start
1976-01-01
Project End
1985-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37203

  Publications

Chan, John; Mehta, Simren; Bharrhan, Sushma et al. (2014) The role of B cells and humoral immunity in Mycobacterium tuberculosis infection. Semin Immunol 26:588-600
Zsak, L; Sugg, N; Ben-Porat, T (1992) The different interactions of a gIII mutant of pseudorabies virus with several different cell types. J Gen Virol 73 ( Pt 4):821-7
Zsak, L; Zuckermann, F; Sugg, N et al. (1992) Glycoprotein gI of pseudorabies virus promotes cell fusion and virus spread via direct cell-to-cell transmission. J Virol 66:2316-25
Kupershmidt, S; Rall, G F; Lu, Z Q et al. (1992) Cleavage of concatemeric DNA at the internal junction of ""translocation"" mutants of pseudorabies virus and inversion of their L component appear to be linked. Virology 187:223-32
Rall, G F; Kupershmidt, S; Sugg, N et al. (1992) Functions of the sequences at the ends of the inverted repeats of pseudorabies virus. J Virol 66:1506-19
Reilly, L M; Rall, G; Lomniczi, B et al. (1991) The ability of pseudorabies virus to grow in different hosts is affected by the duplication and translocation of sequences from the left end of the genome to the UL-US junction. J Virol 65:5839-47
Rall, G F; Lu, Z Q; Sugg, N et al. (1991) Acquisition of an additional internal cleavage site differentially affects the ability of pseudorabies virus to multiply in different host cells. J Virol 65:6604-11
Zsak, L; Sugg, N; Ben-Porat, T et al. (1991) The gIII glycoprotein of pseudorabies virus is involved in two distinct steps of virus attachment. J Virol 65:4317-24
Kupershmidt, S; DeMarchi, J M; Lu, Z Q et al. (1991) Analysis of an origin of DNA replication located at the L terminus of the genome of pseudorabies virus. J Virol 65:6283-91
Rall, G F; Kupershmidt, S; Lu, X Q et al. (1991) Low-level inversion of the L component of pseudorabies virus is not dependent on sequence homology. J Virol 65:7016-9

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