Abstract

The aim of the project is to understand the way the movement of a parasitic nematode is controlled. For the control of nematodes, which are important human parasites, a detailed knowledge of their fundamental physiological mechanisms will enable new drugs to be designed. We propose to study the motor nervous system of Ascaris in order to understand how the output signals to muscles are generated and coordinated to produce locomotion. We will use anatomical, pharmacological and physiological techniques to achieve this. To increase the spectrum of drugs available to paralyzenematodes we will also attempt to identify compounds that selectively inhibit different classes of nerve cells in the system.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI015429-12
Application #
3126165
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1979-01-01
Project End
1994-12-31
Budget Start
1990-01-01
Budget End
1990-12-31
Support Year
12
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
Schools of Arts and Sciences
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Konop, Christopher J; Knickelbine, Jennifer J; Sygulla, Molly S et al. (2015) Mass Spectrometry of Single GABAergic Somatic Motorneurons Identifies a Novel Inhibitory Peptide, As-NLP-22, in the Nematode Ascaris suum. J Am Soc Mass Spectrom 26:2009-23
Konop, Christopher J; Knickelbine, Jennifer J; Sygulla, Molly S et al. (2015) Different neuropeptides are expressed in different functional subsets of cholinergic excitatory motorneurons in the nematode Ascaris suum. ACS Chem Neurosci 6:855-70
Jarecki, Jessica L; Viola, India R; Andersen, Kari M et al. (2013) Three independent techniques localize expression of transcript afp-11 and its bioactive peptide products to the paired AVK neurons in Ascaris suum: in situ hybridization, immunocytochemistry, and single cell mass spectrometry. ACS Chem Neurosci 4:418-34
Reinitz, Catharine A; Pleva, Anthony E; Stretton, Antony O W (2011) Changes in cyclic nucleotides, locomotory behavior, and body length produced by novel endogenous neuropeptides in the parasitic nematode Ascaris suum. Mol Biochem Parasitol 180:27-34
Jarecki, Jessica L; Frey, Brian L; Smith, Lloyd M et al. (2011) Discovery of neuropeptides in the nematode Ascaris suum by database mining and tandem mass spectrometry. J Proteome Res 10:3098-106
Sithigorngul, Paisarn; Jarecki, Jessica L; Stretton, Antony O W (2011) A specific antibody to neuropeptide AF1 (KNEFIRFamide) recognizes a small subset of neurons in Ascaris suum: differences from Caenorhabditis elegans. J Comp Neurol 519:1546-61
Jarecki, Jessica L; Andersen, Kari; Konop, Christopher J et al. (2010) Mapping neuropeptide expression by mass spectrometry in single dissected identified neurons from the dorsal ganglion of the nematode Ascaris suum. ACS Chem Neurosci 1:505-519
Nanda, Jennifer Cho; Stretton, Antony O W (2010) In situ hybridization of neuropeptide-encoding transcripts afp-1, afp-3, and afp-4 in neurons of the nematode Ascaris suum. J Comp Neurol 518:896-910
Yew, Joanne Y; Davis, Ralph; Dikler, Sergei et al. (2007) Peptide products of the afp-6 gene of the nematode Ascaris suum have different biological actions. J Comp Neurol 502:872-82
McKenna, Philip M; Koser, Martin L; Carlson, Kevin R et al. (2007) Highly attenuated rabies virus-based vaccine vectors expressing simian-human immunodeficiency virus89.6P Env and simian immunodeficiency virusmac239 Gag are safe in rhesus macaques and protect from an AIDS-like disease. J Infect Dis 195:980-8

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