Abstract

Research activities in this laboratory are directed towards an understanding of the biology and evolution of extrachromosomal (plasmid) elements in Streptococcus ssp. During the current year, we have identified several different types of tetracycline resistant plasmids from group B Streptococcus. Several of these reside on small non-conjugative plasmids which are capable of transforming S. sanguis Challis. For this reason, they are potentially useful as cloning vehicles in this system. We have cloned two tetracycline determinants in the E. coli system using ColEl-amp as the cloning vehicle. These chimeras have been used to generate a large series of deletions which have given a precise definition of the tetracycline resistance region. In addition, we have used the chimeras to make probes for southern hybridization experiments. These experiments have also led to a better understanding of the tetracycline resistance determinants.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI015619-08
Application #
3126293
Study Section
(MG)
Project Start
1979-01-01
Project End
1987-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
8
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Dantley, K A; Dannelly, H K; Burdett, V (1998) Binding interaction between Tet(M) and the ribosome: requirements for binding. J Bacteriol 180:4089-92
Burdett, V (1996) Tet(M)-promoted release of tetracycline from ribosomes is GTP dependent. J Bacteriol 178:3246-51
Burdett, V (1993) tRNA modification activity is necessary for Tet(M)-mediated tetracycline resistance. J Bacteriol 175:7209-15
Burdett, V (1991) Purification and characterization of Tet(M), a protein that renders ribosomes resistant to tetracycline. J Biol Chem 266:2872-7
Burdett, V (1990) Nucleotide sequence of the tet(M) gene of Tn916. Nucleic Acids Res 18:6137
Miller, L; Burdett, V; Poirier, T P et al. (1988) Conservation of protective and nonprotective epitopes in M proteins of group A streptococci. Infect Immun 56:2198-204
Mouw, A R; Beachey, E H; Burdett, V (1988) Molecular evolution of streptococcal M protein: cloning and nucleotide sequence of the type 24 M protein gene and relation to other genes of Streptococcus pyogenes. J Bacteriol 170:676-84
Haanes-Fritz, E; Kraus, W; Burdett, V et al. (1988) Comparison of the leader sequences of four group A streptococcal M protein genes. Nucleic Acids Res 16:4667-77
McMurry, L M; Park, B H; Burdett, V et al. (1987) Energy-dependent efflux mediated by class L (tetL) tetracycline resistance determinant from streptococci. Antimicrob Agents Chemother 31:1648-50
Burdett, V (1986) Streptococcal tetracycline resistance mediated at the level of protein synthesis. J Bacteriol 165:564-9

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