Abstract

Previous results from our laboratory have provided a method for subclassification of Haemophilus influenzae type b (Hib) based on heterogeneity in the outer membrane proteins (OMP) of different isolates. We propose next to investigate the role of OMP and lipopolysaccharide (LPS) in immunity to this organism. OMP and lipopolysaccharide will be purified from different strains of Hib and characterized using chemical and immunologic methods. The purified derivatives will then be used to develop sensitive and specific serologic techniques for measurement of antibody. Rabbit antiserum will be prepared to purified proteins, and to various LPS preparations, and tested for in vitro bactericidal activity, and for protection against Hib infection in infant rats. The immunogenicity of the different components of the outer membrane will also be examined in humans by measurement of antibody concentrations in serum samples obtained during and after invasive Hib infections. The results of these studies should help determine whether OMP or LPS constituents might be useful for incorporation in an Haemophilus influenzae type b vaccine.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI017572-05
Application #
3127284
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1980-07-01
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Yang, Y P; Munson Jr, R S; Grass, S et al. (1997) Effect of lipid modification on the physicochemical, structural, antigenic and immunoprotective properties of Haemophilus influenzae outer membrane protein P6. Vaccine 15:976-87
Bell, J; Grass, S; Jeanteur, D et al. (1994) Diversity of the P2 protein among nontypeable Haemophilus influenzae isolates. Infect Immun 62:2639-43
Munson Jr, R S; Sasaki, K (1993) Protein D, a putative immunoglobulin D-binding protein produced by Haemophilus influenzae, is glycerophosphodiester phosphodiesterase. J Bacteriol 175:4569-71
Munson Jr, R S; Grass, S; West, R (1993) Molecular cloning and sequence of the gene for outer membrane protein P5 of Haemophilus influenzae. Infect Immun 61:4017-20
Munson Jr, R; Brodeur, B; Chong, P et al. (1992) Outer membrane proteins P1 and P2 of Haemophilus influenzae type b: structure and identification of surface-exposed epitopes. J Infect Dis 165 Suppl 1:S86-9
Coleman, T; Grass, S; Munson Jr, R (1991) Molecular cloning, expression, and sequence of the pilin gene from nontypeable Haemophilus influenzae M37. Infect Immun 59:1716-22
Munson Jr, R; Grass, S; Bailey, C (1991) Comparison of the structure of the genes for outer membrane proteins P1 and P2 of Haemophilus influenzae type b. Mol Immunol 28:257-9
Nelson, M B; Munson Jr, R S; Apicella, M A et al. (1991) Molecular conservation of the P6 outer membrane protein among strains of Haemophilus influenzae: analysis of antigenic determinants, gene sequences, and restriction fragment length polymorphisms. Infect Immun 59:2658-63
Martin, D; Munson Jr, R; Grass, S et al. (1991) Mapping of B-cell epitopes on the outer membrane P2 porin protein of Haemophilus influenzae by using recombinant proteins and synthetic peptides. Infect Immun 59:1457-64
Proulx, C; Munson Jr, R S; Grass, S et al. (1991) Identification of a surface-exposed immunodominant epitope on outer membrane protein P1 of Haemophilus influenzae type b. Infect Immun 59:963-70

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