Abstract

This is an application for study of cell-mediated immune responses to antigens encoded on the murine 17th chromosome outside, but adjacent to, the H-2 complex as traditionally defined. The major portion of this work will focus on gene products of the Qa-1/Tla region. These molecules, including the thymus leukemia antigen, appear most likely to function as differentiation antigens both in normal lymphocyte ontogeny and functional diversification of lymphocyte subsets, and in the development of T cell leukemias and lymphomas. We will extend our ongoing studies on H-2 nonrestricted T cell-mediated cytotoxic reactions induced by antigens of this region. A library of cytotoxic T cell clones with specificy for these determinants will be developed. Antigenic structures and polymorphisms recognized by cytotoxic T cells will be compared with those defined serologically with conventional and monoclonal antibodies. The role of H-2 genes in expression of Qa-1 antigens and in the control of cytotoxic T cell responses to Qa-1 will be defined. We will investigate regulation of T cell responses to Qa-1 antigens in autoimmune NZB mice and normal mice, with particular interest in defining physiological control processes. Receptor specificity for Qa-1 recognition by T cells will be compared with that for recognition for major histocompabitility complex antigens and for recognition of conventional antigens.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI018882-03
Application #
3128283
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1983-04-01
Project End
1988-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Doyle, C Kuyler; Cook, Richard G; Rich, Robert R et al. (2003) Cotton rat Sihi-M3 is a minimally oligomorphic Mhc I-b molecule that binds the chemotactic peptide fMLF under stringent conditions. Evidence that positive selection drives inter-species diversity of residues interacting with the termini of short peptides. Immunogenetics 55:389-94
Davis, Beckley K; Cook, Richard G; Rich, Robert R et al. (2002) Hyperconservation of the putative antigen recognition site of the MHC class I-b molecule TL in the subfamily Murinae: evidence that thymus leukemia antigen is an ancient mammalian gene. J Immunol 169:6890-9
Levitt, J M; Howell, D D; Rodgers, J R et al. (2001) Exogenous peptides enter the endoplasmic reticulum of TAP-deficient cells and induce the maturation of nascent MHC class I molecules. Eur J Immunol 31:1181-90
Howell, D; Levitt, J M; Foster, P A et al. (2000) Heterogeneity of RMA-S cell line: derivatives of RMA-S cells lacking H2-Kb and H2-Db expression. Immunogenetics 52:150-4
Dow, S W; Roberts, A; Vyas, J et al. (2000) Immunization with f-Met peptides induces immune reactivity against Mycobacterium tuberculosis. Tuber Lung Dis 80:13-May
Vyas, J M; Rodgers, J R; Rich, R R (1995) H-2M3a violates the paradigm for major histocompatibility complex class I peptide binding. J Exp Med 181:1817-25
Shawar, S M; Rich, R R; Becker, E L (1995) Peptides from the amino-terminus of mouse mitochondrially encoded NADH dehydrogenase subunit 1 are potent chemoattractants. Biochem Biophys Res Commun 211:812-8
Shawar, S M; Vyas, J M; Rodgers, J R et al. (1994) Antigen presentation by major histocompatibility complex class I-B molecules. Annu Rev Immunol 12:839-80
Vyas, J M; Rich, R R; Howell, D D et al. (1994) Availability of endogenous peptides limits expression of an M3a-Ld major histocompatibility complex class I chimera. J Exp Med 179:155-65
Shawar, S M; Vyas, J M; Shen, E et al. (1993) Differential amino-terminal anchors for peptide binding to H-2M3a or H-2Kb and H-2Db. J Immunol 151:201-10

Showing the most recent 10 out of 28 publications