The overall objective of this project is to better understand novel cellular and molecular aspects of adaptive and innate immune responses in the channel catfish (Ictalurus puntatus), an immunologically well-characterized teleost model system of considerable scientific and economic importance.
The first aim of the proposed studies deals with understanding the complex genetic organization of the teleost IgH locus, which encodes various IgD isotypes from different genes within the locus. The different IgD forms will be studied with respect to their structure, transcriptional regulation, and role in teleost adaptive immune responses. In addition, the mechanism by which thioredoxin activates teleost B cells will be investigated with regard to cellular expression of IgM and IgD isotypes.
The second aim focuses on a diversified V region containing family of novel immune-type receptors (NITR) that exhibit structural diversity with respect to number of extracellular domains, and presence of cytoplasmic activating or inhibitory motifs. Studies are proposed to investigate whether or not teleost NITR molecules have functions analogous to the various receptor products encoded within the human leukocyte receptor complex. These studies include assessment of expression, target recognition, and regulation of innate immune responses. The proposed work is expected to yield novel information concerning the functional roles these potentially important, but poorly understood, molecules play in teleost immune responses. Furthermore, these molecules may prove important in understanding the more fundamental aspects of the evolutionary divergence of adaptive and innate immune functions.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019530-22
Application #
6888053
Study Section
Special Emphasis Panel (ZRG1-IMB (01))
Program Officer
Gondre-Lewis, Timothy A
Project Start
1983-07-01
Project End
2007-05-31
Budget Start
2005-06-01
Budget End
2006-05-31
Support Year
22
Fiscal Year
2005
Total Cost
$333,000
Indirect Cost
Name
University of Mississippi Medical Center
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
928824473
City
Jackson
State
MS
Country
United States
Zip Code
39216
Edholm, Eva-Stina; Bengtén, Eva; Stafford, James L et al. (2010) Identification of two IgD+ B cell populations in channel catfish, Ictalurus punctatus. J Immunol 185:4082-94
Nayak, Deepak K; Tang, Aihua; Wilson, Melanie et al. (2010) Channel catfish soluble FcmuR binds conserved linear epitopes present on Cmu3 and Cmu4. Mol Immunol 47:1306-16
Richard, Mara L Lennard; Hikima, Jun-ichi; Wilson, Melanie R et al. (2009) BOB.1 of the channel catfish, Ictalurus punctatus: not a transcriptional coactivator? Mol Immunol 46:481-91
Edholm, Eva-Stina; Wilson, Melanie; Sahoo, Manoranjan et al. (2009) Identification of Igsigma and Iglambda in channel catfish, Ictalurus punctatus, and Iglambda in Atlantic cod, Gadus morhua. Immunogenetics 61:353-70
Moulana, Mohadetheh; Evenhuis, Jason; Albertino, Mark et al. (2008) Characterization of anti-channel catfish MHC class IIbeta monoclonal antibodies. Vet Immunol Immunopathol 126:120-30
Sahoo, Manoranjan; Edholm, Eva-Stina; Stafford, James L et al. (2008) B cell receptor accessory molecules in the channel catfish, Ictalurus punctatus. Dev Comp Immunol 32:1385-97
Hikima, Jun-ichi; Lennard Richard, Mara L; Wilson, Melanie R et al. (2008) Function of E-protein dimers expressed in catfish lymphocytes. Mol Immunol 45:1165-70
Stafford, James L; Bengten, Eva; Du Pasquier, Louis et al. (2007) Channel catfish leukocyte immune-type receptors contain a putative MHC class I binding site. Immunogenetics 59:77-91
Lennard, Mara L; Hikima, Jun-ichi; Ross, David A et al. (2007) Characterization of an Oct1 orthologue in the channel catfish, Ictalurus punctatus: a negative regulator of immunoglobulin gene transcription? BMC Mol Biol 8:8
Evenhuis, Jason; Bengten, Eva; Snell, Cecile et al. (2007) Characterization of additional novel immune type receptors in channel catfish, Ictalurus punctatus. Immunogenetics 59:661-71

Showing the most recent 10 out of 64 publications