We propose to study the nature of the T-cell recognition structures visualized by allogeneic killer T cells and virus-restricted killer T cells. We will use in vitro mutagenesis to delete, shuffle, and mutate the exons encoding a classical transplantation antigen, the Ld molecule. These mutant forms of the Ld gene will then be transferred into mouse L cells, teratocarcinoma cells, and hopefully into normal bone marrow cells. The ability of the mutated Ld molecule to mediate T-cell killing, to interact with B2-microglobulin, and to interact with virus antigens will then be assessed to shed insights into the natures of the interactions among these molecules in the T-cell recognition structure. We also plan to study the DNA elements which control the expression of several class I genes in various cell lines and, if possible, in mice. Again the class I genes will be altered by in vitro mutagenesis and their expression assessed after appropriate gene transfer experiments.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019624-04
Application #
3128958
Study Section
Immunobiology Study Section (IMB)
Project Start
1983-01-01
Project End
1987-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost
Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125
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Chiang, Eugene Y; Stroynowski, Iwona (2004) A nonclassical MHC class I molecule restricts CTL-mediated rejection of a syngeneic melanoma tumor. J Immunol 173:4394-401
Chiang, Eugene Y; Henson, Maile; Stroynowski, Iwona (2003) Correction of defects responsible for impaired Qa-2 class Ib MHC expression on melanoma cells protects mice from tumor growth. J Immunol 170:4515-23
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Chiang, Eugene Y; Henson, Maile; Stroynowski, Iwona (2002) The nonclassical major histocompatibility complex molecule Qa-2 protects tumor cells from NK cell- and lymphokine-activated killer cell-mediated cytolysis. J Immunol 168:2200-11
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