Abstract

This project deals with the immunobiology and immunogenetics of the HLA system and its clinical applications. Studies are conducted to identify and characterize B cell specific alloantisera and to detect DR antigens and other antigens controlled by linked genes. Of particular significance is a newly defined MB system which is closely associated with HLA-DR. The role of these cell markers in mixed leukocyte culture activation and the generation of cytolytic, memory and suppressor cells is studied. We will also study the effect of MB compatibility on kidney transplant survival. Studies are conducted to define the determinants controlled by the HLA-D locus and their relationship with HLA-DR and MB. Investigations of HLA and disease associations deal with insulin-dependent diabetes of Black patients, acute lymphoblastic leukemia, and ocular histoplasmosis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI021410-02
Application #
3131502
Study Section
Immunobiology Study Section (IMB)
Project Start
1984-01-01
Project End
1986-06-30
Budget Start
1985-01-01
Budget End
1986-06-30
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
Schools of Medicine
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Mangan, K F; Zeevi, A; Duquesnoy, R et al. (1988) A functional analysis of hematopoietic growth factor production from class I and class II human alloreactive T cell clones. Int J Cell Cloning 6:324-40
Stamenkovic, I; Stegagno, M; Wright, K A et al. (1988) Clonal dominance among T-lymphocyte infiltrates in arthritis. Proc Natl Acad Sci U S A 85:1179-83
Trucco, M; Cascino, I; Rosenshine, S et al. (1987) Definition of HLA-DQ polymorphisms by RFLP and in serological and cellular assays. Transplant Proc 19:848-50
Markus, B H; Fung, J J; Zeevi, A et al. (1987) Analysis of T lymphocytes infiltrating human hepatic allografts. Transplant Proc 19:2470-3
Fung, J J; Zeevi, A; Markus, B et al. (1986) Dynamics of allospecific T lymphocyte infiltration in vascularized human allografts. Immunol Res 5:149-63
MacDonald, M J; Famuyiwa, O O; Nwabuebo, I A et al. (1986) HLA-DR associations in black type I diabetics in Nigeria. Further support for models of inheritance. Diabetes 35:583-9
Cascino, I; Rosenshine, S; Turco, E et al. (1986) Relationship between DQ alpha and DQ beta RFLP and cell surface polymorphisms of class II HLA antigens. J Immunogenet 13:387-400
Rosenshine, S; Cascino, I; Zeevi, A et al. (1986) DQ alpha and beta RFLP reveals the composition of the DQ molecule recognized by T-cell clones. Immunogenetics 23:187-96
Fung, J J; Zeevi, A; Starzl, T E et al. (1986) Functional characterization of infiltrating T lymphocytes in human hepatic allografts. Hum Immunol 16:182-99
Trucco, M; Rosenshine, S; Cascino, I et al. (1986) HLA-DQ polymorphism analyzed by sequential restriction endonuclease DNA digestion. Immunogenetics 24:184-90

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