Abstract

The overall objective of this research program is to develop a clear understanding of carbohydrate metabolism in parasitic helminths. Inherent in this objective is the delineation of the regulatory steps which control the flow of carbon through this pathway. A complete description of these regulatory steps and their modulators would provide unique information on the parasite and the manner in which it relates to its environment, the host.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI024155-15
Application #
6169893
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Program Officer
Fairfield, Alexandra
Project Start
1986-04-01
Project End
2004-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
15
Fiscal Year
2000
Total Cost
$239,432
Indirect Cost

  Institution

Name
University of North Texas
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
110091808
City
Fort Worth
State
TX
Country
United States
Zip Code
76107

  Publications

Gibson, Grant E; Harris, Ben G; Cook, Paul F (2006) Optimum activity of the phosphofructokinase from Ascaris suum requires more than one metal ion. Biochemistry 45:2453-60
Cervellati, Carlo; Dallocchio, Franco; Bergamini, Carlo M et al. (2005) Role of methionine-13 in the catalytic mechanism of 6-phosphogluconate dehydrogenase from sheep liver. Biochemistry 44:2432-40
Karsten, William E; Liu, Dali; Rao, G S Jagannatha et al. (2005) A catalytic triad is responsible for acid-base chemistry in the Ascaris suum NAD-malic enzyme. Biochemistry 44:3626-35
Kulkarni, Gopal; Sabnis, Nirupama A; Bhat, Kolari S et al. (2005) Cloning and nucleotide sequence of a full-length cDNA encoding Ascaris suum phosphofructokinase. J Parasitol 91:585-90
Kulkarni, Gopal; Sabnis, Nirupama A; Harris, Ben G (2004) Cloning, expression, and purification of fumarase from the parasitic nematode Ascaris suum. Protein Expr Purif 33:209-13
Rao, G S Jagannatha; Coleman, David E; Karsten, William E et al. (2003) Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site. J Biol Chem 278:38051-8
Karsten, William E; Pais, June E; Rao, G S Jagannatha et al. (2003) Ascaris suum NAD-malic enzyme is activated by L-malate and fumarate binding to separate allosteric sites. Biochemistry 42:9712-21
Coleman, David E; Rao, G S Jagannatha; Goldsmith, E J et al. (2002) Crystal structure of the malic enzyme from Ascaris suum complexed with nicotinamide adenine dinucleotide at 2.3 A resolution. Biochemistry 41:6928-38
Wariso, B A; Harris, B G (2000) Determination of metabolite and regulatory enzyme levels in Dirofilaria immitis and Ascaris suum: a comparative study. West Afr J Med 19:250-3
Jagannatha Rao, G S; Cook, P F; Harris, B G (1999) Kinetic characterization of a T-state of Ascaris suum phosphofructokinase with heterotropic negative cooperativity by ATP eliminated. Arch Biochem Biophys 365:335-43

Showing the most recent 10 out of 37 publications