The goal of the next five years is to understand the molecular basis for the cyclic activity of the fat body of the mosquito, Aedes aegypti. Previously they have succeeded in dissecting the biosynthetic pathway of vitellogenin (Vg) and discovered a novel vitellogenic protein 53KP. The goal for the next five years is to study the regulation of expression of several key genes in the fat body. To that end, cloning of full length cDNAs corresponding to these genes will be obtained. Using these cDNA probes, together with nuclear run on transcription and dot blot hybridization assays, the kinetics and regulation of gene expression will be investigated. A combination of in vivo and in vitro hormone treatments of fat body will be utilized to determine the role of 2O-hydroxyecdysone in the initiation and maintenance of expression of the genes and in the stabilization of their mRNAs, and to identify factors responsible for the termination of gene expression. Biochemical and biosynthetic characterization of yolk protein precursors Vg and 53KP will continue.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI024716-08
Application #
2062696
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Project Start
1986-09-01
Project End
1996-02-29
Budget Start
1994-03-01
Budget End
1995-02-28
Support Year
8
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Michigan State University
Department
Zoology
Type
Schools of Arts and Sciences
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824
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