: gamma/deltaT cells contribute to host immune competence distinctly, but their function remains undefined. Our last granting period yielded the first description of a gamma/deltaT cell population that could potentially mediate immune regulation through cross-talk between lymphocytes and immune protection through clearing infected cells with an activated phenotype. For these studies, we generated a monoclonal antibody specific for the non-classical MHC class I molecule T10, and a related molecule T22, as well as a tetrameric T10/T22 specific gamma/deltaT cell staining reagent. We showed that the expression of T10/T22 is activation induced and that they are ligands for a sizable population (about 0.1-2%) of gamma/delta T cells in unimmunized mice. Our goal is to use this system to study (i) the molecular basis of gamma/delta TCR-T10/T22 interactions; (ii) how this self-reactive TCR repertoire is regulated by ligand expression; and (iii) how T10/T22 specific gamma/delta T cells fit into the context of an immune response to Influenza virus infection. A clear understanding of how a useful gamma/delta TCR repertoire is regulated and how the T10/T22 specificity fits into the context of an immune response will shed new light on the role of these cells in the immune system. Moreover, as the T10/T22 specificity has many of the features expected of regulatory T cells, elucidation of the molecular mechanisms governing their activities should prove relevant to immunity in the contexts of tumor surveillance, autoaggression, and infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI033431-13
Application #
6874428
Study Section
Immunobiology Study Section (IMB)
Program Officer
Rothermel, Annette L
Project Start
1993-01-01
Project End
2008-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
13
Fiscal Year
2005
Total Cost
$360,071
Indirect Cost
Name
Stanford University
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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