Abstract

Baculoviruses are highly virulent insect viruses that serve as biological control agents and as important expression vectors. As pathogenic insect viruses, baculoviruses serve as excellent model systems for studies of viral interactions with insect tissues and cells. Our studies have focused on the major envelope protein, GP64, of the budded virus particle. The GP64 protein appears to serve a number of functions in the infection cycle: 1) recognition of the host cell receptor molecules; 2) fusion of viral and host membranes during virus entry; and, 3) participation in budding or assembly of the virus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI033657-08
Application #
6373310
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Meegan, James M
Project Start
1993-08-01
Project End
2004-03-31
Budget Start
2001-04-01
Budget End
2002-03-31
Support Year
8
Fiscal Year
2001
Total Cost
$163,633
Indirect Cost

  Institution

Name
Boyce Thompson Institute for Plant Research
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14853

  Publications

Chen, Yun-Ru; Zhong, Silin; Fei, Zhangjun et al. (2013) The transcriptome of the baculovirus Autographa californica multiple nucleopolyhedrovirus in Trichoplusia ni cells. J Virol 87:6391-405
Dong, Sicong; Blissard, Gary W (2012) Functional analysis of the Autographa californica multiple nucleopolyhedrovirus GP64 terminal fusion loops and interactions with membranes. J Virol 86:9617-28
Li, Zhaofei; Blissard, Gary W (2012) Cellular VPS4 is required for efficient entry and egress of budded virions of Autographa californica multiple nucleopolyhedrovirus. J Virol 86:459-72
Li, Zhaofei; Blissard, Gary W (2011) Autographa californica multiple nucleopolyhedrovirus GP64 protein: roles of histidine residues in triggering membrane fusion and fusion pore expansion. J Virol 85:12492-504
Hashimoto, Yoshifumi; Zhang, Sheng; Blissard, Gary W (2010) Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins. BMC Biotechnol 10:50
Li, Zhaofei; Blissard, Gary W (2010) Baculovirus GP64 disulfide bonds: the intermolecular disulfide bond of Autographa californica multicapsid nucleopolyhedrovirus GP64 is not essential for membrane fusion and virion budding. J Virol 84:8584-95
Li, Zhaofei; Blissard, Gary W (2009) The pre-transmembrane domain of the Autographa californica multicapsid nucleopolyhedrovirus GP64 protein is critical for membrane fusion and virus infectivity. J Virol 83:10993-1004
Li, Zhaofei; Blissard, Gary W (2009) The Autographa californica multicapsid nucleopolyhedrovirus GP64 protein: analysis of transmembrane domain length and sequence requirements. J Virol 83:4447-61
Li, Zhaofei; Blissard, Gary W (2008) Functional analysis of the transmembrane (TM) domain of the Autographa californica multicapsid nucleopolyhedrovirus GP64 protein: substitution of heterologous TM domains. J Virol 82:3329-41
Zhou, Jian; Blissard, Gary W (2008) Display of heterologous proteins on gp64null baculovirus virions and enhanced budding mediated by a vesicular stomatitis virus G-stem construct. J Virol 82:1368-77

Showing the most recent 10 out of 15 publications