Abstract

. This second resubmission entitled """"""""Immunodominant Targets of CMI to Cryptosporidium"""""""" is to identify important cryptosporidial antigens in immune response and recovery. The applicant proposes to identify and isolate recombinant C. parvum antigens from a cDNA library using polyclonal and monoclonal antibodies generated against cryptosporidial proteins, to evaluate purified recombinant proteins for their immunogenicity using a T cell proliferation assay as either gel purified protein or a nitrocellulose- bound protein (T cell immunoblot assay) in order to determine whether these antigens elicit T cell responses and finally to isolate T cell clones that elicit strong responses to these antigens and then engraft them to infected SCID mice to determine whether these can reduce parasite load or induce recovery.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI036680-02
Application #
2413695
Study Section
AIDS and Related Research Study Section 5 (ARRE)
Project Start
1996-05-01
Project End
1999-04-30
Budget Start
1997-05-01
Budget End
1998-04-30
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Emory University
Department
Pediatrics
Type
Schools of Medicine
DUNS #
042250712
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

McNair, Nina N; Mead, Jan R (2013) CD4? effector and memory cell populations protect against Cryptosporidium parvum infection. Microbes Infect 15:599-606
Bedi, B; Mead, J R (2012) Cryptosporidium parvum antigens induce mouse and human dendritic cells to generate Th1-enhancing cytokines. Parasite Immunol 34:473-85
Benitez, Alvaro; Priest, Jeffrey W; Ehigiator, Humphrey N et al. (2011) Evaluation of DNA encoding acidic ribosomal protein P2 of Cryptosporidium parvum as a potential vaccine candidate for cryptosporidiosis. Vaccine 29:9239-45
Mead, Jan R (2010) Challenges and prospects for a Cryptosporidium vaccine. Future Microbiol 5:335-7
Benitez, Alvaro J; McNair, Nina; Mead, Jan R (2009) Oral immunization with attenuated Salmonella enterica serovar Typhimurium encoding Cryptosporidium parvum Cp23 and Cp40 antigens induces a specific immune response in mice. Clin Vaccine Immunol 16:1272-8
Ehigiator, Humphrey N; Romagnoli, Pablo; Priest, Jeffrey W et al. (2007) Induction of murine immune responses by DNA encoding a 23-kDa antigen of Cryptosporidium parvum. Parasitol Res 101:943-50
Ehigiator, Humphrey N; McNair, Nina; Mead, Jan R (2007) Cryptosporidium parvum: the contribution of Th1-inducing pathways to the resolution of infection in mice. Exp Parasitol 115:107-13
Ehigiator, H N; Romagnoli, P; Borgelt, K et al. (2005) Mucosal cytokine and antigen-specific responses to Cryptosporidium parvum in IL-12p40 KO mice. Parasite Immunol 27:17-28
Smith, L M; Bonafonte, M T; Mead, J R (2000) Cytokine expression and specific lymphocyte proliferation in two strains of Cryptosporidium parvum-infected gamma-interferon knockout mice. J Parasitol 86:300-7
Bonafonte, M T; Smith, L M; Mead, J R (2000) A 23-kDa recombinant antigen of Cryptosporidium parvum induces a cellular immune response on in vitro stimulated spleen and mesenteric lymph node cells from infected mice. Exp Parasitol 96:32-41

Showing the most recent 10 out of 17 publications