The overall goal of these studies is to gain further insight into the role of second receptors during primate immunodeficiency virus entry into cells. The experimental goals are broken down into four categories. In the first set of experiments, the interaction between HIV-1 gp120 and various second receptors will be studied. These studies involve extensive mutagenesis of gp120, CKR5 and fusin.
The second Aim i s to clone and characterize the second receptors for HIV-2 and non human primate immunodeficiency viruses from chimpanzee, macaque and mangabey as well as from human PBMC.
The third Aim will evaluate the ability of HIV strains from different subtypes to utilize different second receptors.
The fourth Aim i s to understand whether and how variation in the second receptor expression patterns in macrophages and lymphocytes influences HIV-1 entry into these cells; variations in the sensitivity of infection to blocking by b-chemokines will also be examined.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI041420-01
Application #
2330567
Study Section
AIDS and Related Research Study Section 3 (ARRC)
Project Start
1997-07-01
Project End
2001-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Aaron Diamond AIDS Research Center
Department
Type
DUNS #
786658872
City
New York
State
NY
Country
United States
Zip Code
10016
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