Abstract

The long term objective of this project is to isolate, characterize and clone carbonic anhydrase and chloride/bicarbonate anion exchanger from the disease vector mosquito, Aedes aegypti, and the transport model caterpillar, Manduca sexta. The role of theses two enzymes in midgut alkalinzation and ionic homeostasis will be determined and models of anionic buffer regulation will be formulated. The isolated proteinscDNAs will be used to prepare immunological and molecular probes for cellular localization analyses. The hupothesies is that the uniquely high alkalinity of the midgut contents is achieved across an H+ V-ATPase-hyperpolarization membrane by electrophoretic exchange of luminal H+ for cell K+, substrate-driven exchange of luminal Cl- for HCO3-, and voltagew driven removal of H+ to yield CO3--. Since, carbonic anhydrase and anion exchanger inhibition maqy be attractive candidates for novel, environmentally safe, larvacides.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI045098-03
Application #
6349905
Study Section
Special Emphasis Panel (ZRG1-TMP (01))
Program Officer
Aultman, Kathryn S
Project Start
1999-02-01
Project End
2002-01-31
Budget Start
2001-02-01
Budget End
2002-01-31
Support Year
3
Fiscal Year
2001
Total Cost
$203,475
Indirect Cost

  Institution

Name
University of Florida
Department
Type
Organized Research Units
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611

  Publications

White, Bradley J; Kundert, Peter N; Turissini, David A et al. (2013) Dose and developmental responses of Anopheles merus larvae to salinity. J Exp Biol 216:3433-41
Sterling, Kenneth M; Okech, Bernard A; Xiang, Minghui A et al. (2012) High affinity (3)H-phenylalanine uptake by brush border membrane vesicles from whole larvae of Aedes aegypti (AaBBMVw). J Insect Physiol 58:580-9
Hirata, Taku; Czapar, Anna; Brin, Lauren et al. (2012) Ion and solute transport by Prestin in Drosophila and Anopheles. J Insect Physiol 58:563-9
Linser, Paul J; Neira Oviedo, Marco; Hirata, Taku et al. (2012) Slc4-like anion transporters of the larval mosquito alimentary canal. J Insect Physiol 58:551-62
Xiang, Minghui A; Linser, Paul J; Price, David A et al. (2012) Localization of two Na+- or K+-H+ antiporters, AgNHA1 and AgNHA2, in Anopheles gambiae larval Malpighian tubules and the functional expression of AgNHA2 in yeast. J Insect Physiol 58:570-9
Smith, Kristin E; Raymond, Steven L; Valenti, Micheala L et al. (2010) Physiological and pharmacological characterizations of the larval Anopheles albimanus rectum support a change in protein distribution and/or function in varying salinities. Comp Biochem Physiol A Mol Integr Physiol 157:55-62
Harvey, William R; Okech, Bernard A; Linser, Paul J et al. (2010) H(+) V-ATPase-energized transporters in brush border membrane vesicles from whole larvae of Aedes aegypti. J Insect Physiol 56:1377-89
Smith, Kristin E; Linser, Paul J (2009) Silencing of carbonic anhydrase in an Anopheles gambiae larval cell line, Ag55. J RNAi Gene Silencing 5:345-50
Linser, Paul J; Smith, Kristin E; Seron, Terri J et al. (2009) Carbonic anhydrases and anion transport in mosquito midgut pH regulation. J Exp Biol 212:1662-71
Neira Oviedo, M; Ribeiro, J M C; Heyland, A et al. (2009) The salivary transcriptome of Anopheles gambiae (Diptera: Culicidae) larvae: A microarray-based analysis. Insect Biochem Mol Biol 39:382-94

Showing the most recent 10 out of 18 publications