Abstract

Our overall objective is to characterize the cell surface membrane complex, using flourescence spectroscopy and microscopy in conjunction with detailed cytological, biochemical, and functional analyses. The proposed experiments are designed to test our previous hypothesis that there is a cell surface modulating assembly that can control the mobility of surface receptors and that can influence cell growth, motility, and interaction with other cells. In particular, we will use fluorescence photobleaching recovery and biochemical methods to measure quantitatively the mobility of cell surface receptors and to identify cytoplasmic components that may interact with surface receptors to form such a modulating assembly.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM004256-26
Application #
3150707
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1976-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
26
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Richardson, G P; Crossin, K L; Chuong, C M et al. (1987) Expression of cell adhesion molecules during embryonic induction. III. Development of the otic placode. Dev Biol 119:217-30
Hoffman, S; Edelman, G M (1987) A proteoglycan with HNK-1 antigenic determinants is a neuron-associated ligand for cytotactin. Proc Natl Acad Sci U S A 84:2523-7
Gallin, W J; Sorkin, B C; Edelman, G M et al. (1987) Sequence analysis of a cDNA clone encoding the liver cell adhesion molecule, L-CAM. Proc Natl Acad Sci U S A 84:2808-12
Edelman, G M (1987) Epigenetic rules for expression of cell adhesion molecules during morphogenesis. Ciba Found Symp 125:192-216
Chuong, C M; Crossin, K L; Edelman, G M (1987) Sequential expression and differential function of multiple adhesion molecules during the formation of cerebellar cortical layers. J Cell Biol 104:331-42
Cunningham, B A; Hemperly, J J; Murray, B A et al. (1987) Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. Science 236:799-806
Levi, G; Crossin, K L; Edelman, G M (1987) Expression sequences and distribution of two primary cell adhesion molecules during embryonic development of Xenopus laevis. J Cell Biol 105:2359-72
Owens, G C; Edelman, G M; Cunningham, B A (1987) Organization of the neural cell adhesion molecule (N-CAM) gene: alternative exon usage as the basis for different membrane-associated domains. Proc Natl Acad Sci U S A 84:294-8
Edelman, G M (1987) CAMs and Igs: cell adhesion and the evolutionary origins of immunity. Immunol Rev 100:11-45
Murray, B A; Hoffman, S; Cunningham, B A (1987) Molecular features of cell-cell adhesion molecules. Prog Brain Res 71:35-45

Showing the most recent 10 out of 29 publications