Abstract

The objectives of the proposed research constitutes a continuation of investigations on the transport of calcium and other mineral ions across the epithelium of the intestine. Emphasis will be given to different aspects of the problems of vitamin D action.
Specific aims are to: (a) determine the amino acid sequence of chick CaBP and its interactions, (b) investigate in detail alterations in phopholipid metabolism as related to 1,25(OH)2D3 action, (c) to study possible 1,25(OH)2D3-mediated changes in membrane fluidity and mobility of membrane components, (d) initiate studies on the use of epithelial cell lines to investigate aspects of 1,25(OH)2D3 action, (e) investigate the properties and characteristics of the ATP-dependent basal-lateral pump, (f) identify and investigate the properties and function of specific 1,25(OH)2D3-modulated proteins and lipids, (g) examine hormonal effects in a model in which chicks are given 1,25(OH)2D3 as their only source of vitamin D3, and (h) to determine possible alterations in calmodulin behavior in calcium, phosphorus and vitamin D3-deficiency.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM004652-25
Application #
3150711
Study Section
General Medicine B Study Section (GMB)
Project Start
1978-12-01
Project End
1988-11-30
Budget Start
1984-12-01
Budget End
1985-11-30
Support Year
25
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Cornell University
Department
Type
Schools of Veterinary Medicine
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850

  Publications

Corradino, R A; Smith, C A; Krook, L P et al. (1993) Tissue-specific regulation of shell gland calbindin D28K biosynthesis by estradiol in precociously matured, vitamin D-depleted chicks. Endocrinology 132:193-8
Corradino, R A; Fullmer, C S (1991) Positive cotranscriptional regulation of intestinal calbindin-D28K gene expression by 1,25-dihydroxyvitamin D3 and glucocorticoids. Endocrinology 128:944-50
McCormick, C C; Fullmer, C S; Garvey, J S (1988) Amino acid sequence and comparative antigenicity of chicken metallothionein. Proc Natl Acad Sci U S A 85:309-13
Wasserman, R H (1988) Calcium and phosphate entry into cells: a brief overview. Prog Clin Biol Res 252:3-13
Fullmer, C S; Wasserman, R H (1987) Chicken intestinal 28-kilodalton calbindin-D: complete amino acid sequence and structural considerations. Proc Natl Acad Sci U S A 84:4772-6
Fullmer, C S; Edelstein, S; Wasserman, R H (1985) Lead-binding properties of intestinal calcium-binding proteins. J Biol Chem 260:6816-9