Abstract

This proposal is concerned with the determination of the chemical structures of slow-reacting substances (SRS) from cat paws and rat basophilic leukemic (RBL-1) cells, which have now been purified for the first time. The metabolism of arachidonic and 5,8,11,14,17-eicosapentaenoic acids (E.P.A.) in relation to SRS RBL formation will be investigated in RBL-1 tissue culture systems. Radioactive E.P.A. will be prepared to detect the possible existence of novel lipoxygenase pathways in tissue homogenates. PGH3 will be prepared and its decomposition products will be characterized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM009688-20
Application #
3150761
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1975-06-01
Project End
1986-06-30
Budget Start
1984-12-01
Budget End
1986-06-30
Support Year
20
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
Schools of Pharmacy
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Shieh, W R; Cacioli, P; Sih, C J (1985) Mechanisms of inactivation of soybean lipoxygenase by acetylenic fatty acids. Adv Prostaglandin Thromboxane Leukot Res 15:303-6