Abstract

Recent developments in the study of the structure and function of muscle sarcoplasmic reticulum have led to the resolution of molecular components involved in Ca2 ion transport. Very little is known, however, about the mechanism by which Ca2 ion is released from the sarcoplasmic reticulum, which presumably is a key process in muscle contraction. In order to gain further understanding of the molecular mechanism of Ca2 ion transport and release in the sarcoplasmic reticulum, we plan to study the molecular structure and disposition of some membrane components of the protein, viz. the ATPase enzyme, calsequestrin and glycoprotein; and the interaction of these components with phospholipids in an effort to reconstitute vesicles capable of Ca2 ion transport and release under physiological conditions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM016922-13
Application #
3151035
Study Section
Physiology Study Section (PHY)
Project Start
1976-06-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Boston Biomedical Research Institute
Department
Type
DUNS #
058893371
City
Watertown
State
MA
Country
United States
Zip Code

  Publications

Kim, D H; Sreter, F A; Ikemoto, N (1988) Involvement of the 60 kDa phosphoprotein in the regulation of Ca2+ release from sarcoplasmic reticulum of normal and malignant hyperthermia susceptible pig muscles. Biochim Biophys Acta 945:246-52
Ikemoto, N; Kim, D H; Antoniu, B (1988) Measurement of calcium release in isolated membrane systems: coupling between the transverse tubule and sarcoplasmic reticulum. Methods Enzymol 157:469-80
Meszaros, L G; Brown, K L; Ikemoto, N (1987) 4',6-Diamidino-2-phenylindole, a novel conformational probe of the sarcoplasmic reticulum Ca2+ pump, and its effect on Ca2+ release. J Biol Chem 262:11553-8
Kim, H W; Kim, D H; Ikemoto, N et al. (1987) Lack of effects of calcium X calmodulin-dependent phosphorylation on Ca2+ release from cardiac sarcoplasmic reticulum. Biochim Biophys Acta 903:333-40
Kim, D H; Ikemoto, N (1986) Involvement of 60-kilodalton phosphoprotein in the regulation of calcium release from skeletal muscle sarcoplasmic reticulum. J Biol Chem 261:11674-9
Morii, M; Danko, S; Kim, D H et al. (1986) Fluorescence conformational probe study of calcium release from sarcoplasmic reticulum. J Biol Chem 261:2343-8
Meszaros, L G; Ikemoto, N (1985) Ruthenium red and caffeine affect the Ca2+-ATPase of the sarcoplasmic reticulum. Biochem Biophys Res Commun 127:836-42
Danko, S; Kim, D H; Sreter, F A et al. (1985) Inhibitors of Ca2+ release from the isolated sarcoplasmic reticulum. II. The effects of dantrolene on Ca2+ release induced by caffeine, Ca2+ and depolarization. Biochim Biophys Acta 816:18-24
Antoniu, B; Kim, D H; Morii, M et al. (1985) Inhibitors of Ca2+ release from the isolated sarcoplasmic reticulum. I. Ca2+ channel blockers. Biochim Biophys Acta 816:9-17
Meszaros, L G; Ikemoto, N (1985) Conformational changes of the Ca2+-ATPase as early events of Ca2+ release from sarcoplasmic reticulum. J Biol Chem 260:16076-9

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