Abstract

The purpose of the proposed research program is to gain further detailed information into the cellular mechanisms responsible for ion transport by epithelial tissues. These studies will involve (a) the determination of the current-voltage relation of the Na entry step in tight epithelia such as colon, urinary bladder, frog skin, etc., using a new developed and unique computer-driven voltage-clamp apparatus; (b) continued investigations of the intracellular Na, K and Cl thermodynamic activities of epithelial tissues determined using ion-selective microelectrodes in an effort to relate these activities to transcellular ion transport; (c) studies of the current-voltage relation of Na-coupled sugar and amino acid transport by small intestine in an effort to further define the kinetic characteristics of these carrier-mediated processes; and (d) to initiate studies of the transport and electrophysiologic properties of mono-layers of cultured renal and colonic epithelial cells in an effort to circumvent the problems that arise when one is dealing with the """"""""natural"""""""" tissue characterized by an irregular geometry and heterogeneous cell types.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM026690-07
Application #
3151654
Study Section
Physiology Study Section (PHY)
Project Start
1979-09-01
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center Houston
Department
Type
Schools of Medicine
DUNS #
City
Houston
State
TX
Country
United States
Zip Code
77225