Abstract

Hybridoma-produced autoantibodies to DNA will be used to analyze genetic and cellular aspects of experimental systemic lupus erythematosus. These monoclonal autoantibodies, together with corresponding anti-idiotypic antibodies will be employed to quantitate cross reactive idiotypes in serum as well as suppressor T cells, helper T cells and B cells with specificity for autoantibodies to DNA. Experiments will be done in MRL/I mice, which develop a severe and rapidly fatal form of systemic lupus erythematosus. The hybridoma autoantibodies will also be used to study recognition of helical nucleic acids by proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM027232-06
Application #
3151726
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1980-09-25
Project End
1986-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code

  Publications

Schwartz, R S (1988) Polyvalent anti-DNA autoantibodies: immunochemical and biological significance. Int Rev Immunol 3:97-115
Naparstek, Y; Baur, K; Reis, M D et al. (1988) Autoreactive T cells with atypical MHC restriction from MRL-lpr/lpr mice: forbidden clones revisited. J Mol Cell Immunol 4:35-43
Migliorini, P; Ardman, B; Kaburaki, J et al. (1987) Parallel sets of autoantibodies in MRL-lpr/lpr mice. An anti-DNA, anti-SmRNP, anti-gp70 network. J Exp Med 165:483-99
Schwartz, R S (1987) Some speculations on the origins of autoantibodies. Ann N Y Acad Sci 505:8-11
Naparstek, Y; Andre-Schwartz, J; Manser, T et al. (1986) A single germline VH gene segment of normal A/J mice encodes autoantibodies characteristic of systemic lupus erythematosus. J Exp Med 164:614-26
Schwartz, R S (1986) Anti-DNA antibodies and the problem of autoimmunity. Cell Immunol 99:38-43
Stollar, B D (1986) Antibodies to DNA. CRC Crit Rev Biochem 20:1-36
Stollar, B D; Zon, G; Pastor, R W (1986) A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody. Proc Natl Acad Sci U S A 83:4469-73
Stollar, B D (1985) On the nature, origin and clinical significance of anti-DNA autoantibodies. Scand J Rheumatol Suppl 56:22-31
Morgan, A; Isenberg, D A; Naparstek, Y et al. (1985) Shared idiotypes are expressed on mouse and human anti-DNA autoantibodies. Immunology 56:393-9

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