Abstract

Several series of experiments are planned in continuing work on two neural peptides, substance P and neurotensin, that have been isolated, sequenced, and synthesized in this laboratory. The studies outlined include a) mapping the distribution of these peptides in brain and peripheral nerve. b) delineation of substance P or neurotensin-containing tracts within the central nervous system. c) the release of substance P and neurotensin from neural tissue in vitro and in vivo. d) the chemical characterization of substance P and neurotensin in plasma. e) manipulation of the plasma levels of immunoreactive substance P and neurotensin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
5R01AM029876-06
Application #
3151978
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1980-12-01
Project End
1987-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Massachusetts Medical School Worcester
Department
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code

  Publications

Floor, E; Leeman, S E (1988) Identification and characterization of the major proteins of mammalian brain synaptic vesicles. J Neurochem 50:1597-604
Floor, E; Schaeffer, S F; Feist, B E et al. (1988) Synaptic vesicles from mammalian brain: large-scale purification and physical and immunochemical characterization. J Neurochem 50:1588-96
Aronin, N; Leeman, S E; Clements Jr, R S (1987) Diminished flare response in neuropathic diabetic patients. Comparison of effects of substance P, histamine, and capsaicin. Diabetes 36:1139-43
Jonassen, J A; Mullikin-Kilpatrick, D; McAdam, A et al. (1987) Thyroid hormone status regulates preprotachykinin-A gene expression in male rat anterior pituitary. Endocrinology 121:1555-61
Aronin, N; Coslovsky, R; Leeman, S E (1986) Substance P and neurotensin: their roles in the regulation of anterior pituitary function. Annu Rev Physiol 48:537-49
Ferris, C F; Carraway, R E; Brandt, K et al. (1986) Chromatographic and immunochemical characterization of neurotensin in cat adrenal gland and its release during splanchnic nerve stimulation. Neuroendocrinology 43:352-8
Oblas, B; Singer, R H; Boyd, N D (1986) Location of a polypeptide sequence within the alpha-subunit of the acetylcholine receptor containing the cholinergic binding site. Mol Pharmacol 29:649-56
Armstrong, M J; Parker, M C; Ferris, C F et al. (1986) Neurotensin stimulates [3H]oleic acid translocation across rat small intestine. Am J Physiol 251:G823-9
Floor, E; Leeman, S E (1985) Evidence that large synaptic vesicles containing substance P and small synaptic vesicles have a surface antigen in common in rat. Neurosci Lett 60:231-7
Takano, Y; Sawyer, W B; Loewy, A D (1985) Substance P mechanisms of the spinal cord related to vasomotor tone in the spontaneously hypertensive rat. Brain Res 334:105-16

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