Abstract

The principal goal of this project is to utilize dispersed parathyroid cells to study the regulation of parathyroid hormone (PTH) secretion from normal and abnormal parathyroid tissue. The role of cyclic nucleotides in PTH release from dispersed bovine parathyroid cells will be determined by evaluating the effects of divalent cations and diverse secretagogues on adenylate cyclase, phosphodiesterase, and protein kinase activities. Abnormalities in calcium- and secretagogue-stimulated secretion will be assessed in pathologic parathyroid tissue. Possible malregulation of the intracellular content and action of mediators such as cyclic AMP will be studied in terms of alterations in the control of these functions in normal parathyroid cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Project (R01)
Project #
7R01AM036801-01
Application #
3154571
Study Section
General Medicine B Study Section (GMB)
Project Start
1985-07-01
Project End
1987-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code

  Publications

Hawkins, D; Enyedi, P; Brown, E (1989) The effects of high extracellular Ca2+ and Mg2+ concentrations on the levels of inositol 1,3,4,5-tetrakisphosphate in bovine parathyroid cells. Endocrinology 124:838-44
Brent, G A; LeBoff, M S; Seely, E W et al. (1988) Relationship between the concentration and rate of change of calcium and serum intact parathyroid hormone levels in normal humans. J Clin Endocrinol Metab 67:944-50
Kifor, O; Brown, E M (1988) Relationship between diacylglycerol levels and extracellular Ca2+ in dispersed bovine parathyroid cells. Endocrinology 123:2723-9
Chen, C J; Anast, C S; Brown, E M (1988) Effects of fluoride on parathyroid hormone secretion and intracellular second messengers in bovine parathyroid cells. J Bone Miner Res 3:279-88
Chen, C J; Anast, C S; Posillico, J T et al. (1987) Effects of extracellular calcium and magnesium on cytosolic calcium concentration in Fura-2-loaded bovine parathyroid cells. J Bone Miner Res 2:319-27
Chen, C J; Anast, C S; Brown, E M (1987) High osmolality: a potent parathyroid hormone secretogogue in dispersed parathyroid cells. Endocrinology 121:958-64
Posillico, J T; Srikanta, S; Eisenbarth, G et al. (1987) Binding of monoclonal antibody (4F2) to its cell surface antigen on dispersed adenomatous parathyroid cells raises cytosolic calcium and inhibits parathyroid hormone secretion. J Clin Endocrinol Metab 64:43-50
Oetting, M; LeBoff, M S; Levy, S et al. (1987) Permeabilization reveals classical stimulus-secretion coupling in bovine parathyroid cells. Endocrinology 121:1571-6
Brown, E; Enyedi, P; LeBoff, M et al. (1987) High extracellular Ca2+ and Mg2+ stimulate accumulation of inositol phosphates in bovine parathyroid cells. FEBS Lett 218:113-8
Brown, E M; LeBoff, M S; Oetting, M et al. (1987) Secretory control in normal and abnormal parathyroid tissue. Recent Prog Horm Res 43:337-82

Showing the most recent 10 out of 12 publications